DC pole | Wartość | Język |
dc.contributor.author | Swida-Barteczka, Aleksandra | - |
dc.contributor.author | Kruszka, Katarzyna | - |
dc.contributor.author | Grabowska, Aleksandra | - |
dc.contributor.author | Pacak, Andrzej | - |
dc.contributor.author | Jarmolowski, Artur | - |
dc.contributor.author | Kurowska, Marzena | - |
dc.contributor.author | Szarejko, Iwona | - |
dc.contributor.author | Szweykowska-Kulinska, Zofia | - |
dc.date.accessioned | 2019-08-05T07:24:01Z | - |
dc.date.available | 2019-08-05T07:24:01Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Acta Biochimica Polonica, 2016, vol. 63, no. 4, p. 817-824 | pl_PL |
dc.identifier.issn | 0001-527X | - |
dc.identifier.issn | 1734-154X | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/10502 | - |
dc.description.abstract | MicroRNAs are short molecules of 21–24 nt in length.
They are present in all eukaryotic organisms and regulate
gene expression by guiding posttranscriptional
silencing of mRNAs. In plants, they are key players in
signal transduction, growth and development, and in
response to abiotic and biotic stresses. Barley (Hordeum
vulgare) is an economically important monocotyledonous
crop plant. Drought is the world’s main cause of
loss in cereal production. We have constructed a highthroughput
Real-Time RT-qPCR platform for parallel determination
of 159 barley primary microRNAs’ levels. The
platform was tested for two drought-and-rehydrationtreated
barley genotypes (Rolap and Sebastian). We
have determined changes in the expression of primary
microRNAs responding to mild drought, severe drought,
and rehydration. Based on the results obtained, we conclude
that alteration in the primary microRNA expression
is relative to the stress’s intensity. Mild drought and rehydration
mostly decrease the pri-miRNA levels in both
of the tested genotypes. Severe drought mainly induces
the primary microRNA expression. The main difference
between the genotypes tested was a much-stronger induction
of pri-miRNAs in Rolap encountering severe
drought. The primary microRNAs respond dynamically
to mild drought, severe drought, and rehydration treatments.
We propose that some of the individual primiRNAs
could be used as drought stress or rehydration
markers. The usage of the platform in biotechnology is
also postulated. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa-Na tych samych warunkach 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by-sa/3.0/pl/ | * |
dc.subject | Pri-miRNA | pl_PL |
dc.subject | mrRNA | pl_PL |
dc.subject | Drought | pl_PL |
dc.subject | Rehydration | pl_PL |
dc.subject | Barley genotypes | pl_PL |
dc.title | Barley primary microRNA expression pattern is affected by soil water availability | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.18388/abp.2016_1352 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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