DC pole | Wartość | Język |
dc.contributor.author | Szurman-Zubrzycka, Miriam | - |
dc.contributor.author | Nawrot, Małgorzata | - |
dc.contributor.author | Jelonek, Janusz | - |
dc.contributor.author | Dziekanowski, Mariusz | - |
dc.contributor.author | Kwaśniewska, Jolanta | - |
dc.contributor.author | Szarejko, Iwona | - |
dc.date.accessioned | 2019-10-23T13:10:18Z | - |
dc.date.available | 2019-10-23T13:10:18Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | "Frontiers in Plant Science" Vol. 10 (2019), art. no 1299 | pl_PL |
dc.identifier.issn | 1664-462X | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/11424 | - |
dc.description.abstract | Ataxia Telangiectasia and Rad-3-related protein (ATR) is a DNA damage signaling kinase
required for the monitoring of DNA integrity. Together with ATM and SOG1, it is a key
player in the transcriptional regulation of DNA damage response (DDR) genes in plants. In
this study, we describe the role of ATR in the DDR pathway in barley and the function of
the HvATR gene in response to DNA damages induced by aluminum toxicity. Aluminum
is the third most abundant element in the Earth’s crust. It becomes highly phytotoxic in
acidic soils, which comprise more than 50% of arable lands worldwide. At low pH, Al is
known to be a genotoxic agent causing DNA damage and cell cycle arrest. We present
barley mutants, hvatr.g and hvatr.i, developed by TILLING strategy. The hvatr.g mutant
carries a G6054A missense mutation in the ATR gene, leading to the substitution of a
highly conserved amino acid in the protein (G1015S). The hvatr.g mutant showed the
impaired DDR pathway. It accumulated DNA damages in the nuclei of root meristem
cells when grown in control conditions. Terminal deoxynucleotidyl transferase-mediated
dUTP nick-end labeling (TUNEL) analysis revealed that 60% of mutant nuclei possessed
DNA nicks and breaks, whereas in the wild type only 2% of the nuclei were TUNELpositive.
The high frequency of DNA damages did not lead to the inhibition of the cell
cycle progression, but the mutant showed an increased number of cells in the G2/M
phase. In response to treatments with different Al doses, hvatr.g showed a high level
of tolerance. The retention of root growth, which is the most evident symptom of Al
toxicity, was not observed in the mutant, as it was in its parent variety. Furthermore, Al
treatment increased the level of DNA damages, but did not affect the mitotic activity and
the cell cycle profile in the hvatr.g mutant. A similar phenotype was observed for the hvatr.i
mutant, carrying another missense mutation leading to G903E substitution in the HvATR
protein. Our results demonstrate that the impaired mechanism of DNA damage response
may lead to aluminum tolerance. They shed a new light on the role of the ATR-dependent
DDR pathway in an agronomically important species. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | aluminum | pl_PL |
dc.subject | ATR | pl_PL |
dc.subject | DDR pathway | pl_PL |
dc.subject | barley | pl_PL |
dc.subject | TILLING | pl_PL |
dc.title | ATR, a DNA damage signaling kinase, is involved in aluminum response in barley | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.3389/fpls.2019.01299 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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