Skip navigation

Please use this identifier to cite or link to this item:
Title: Comparatively Barcoded Chromosomes of Brachypodium Perennials Tell the Story of Their Karyotype Structure and Evolution
Authors: Lusińska, Joanna
Betekhtin, Alexander
Lopez-Alvarez, Diana
Catalan, Pilar
Jenkins, Glyn
Wolny, Elżbieta
Hasterok, Robert
Keywords: Brachypodium; comparative chromosome barcoding; dysploidy; karyotype structure and evolution; model grass genus;; molecular cytogenetics; polyploidy
Issue Date: 2019
Citation: International Journal of Molecular Sciences, Vol. 20 (2019), Art. No. 5557
Abstract: The Brachypodium genus is an informative model system for studying grass karyotype organization. Previous studies of a limited number of species and reference chromosomes have not provided a comprehensive picture of the enigmatic phylogenetic relationships in the genus. Comparative chromosome barcoding, which enables the reconstruction of the evolutionary history of individual chromosomes and their segments, allowed us to infer the relationships between putative ancestral karyotypes of extinct species and extant karyotypes of current species. We used over 80 chromosome-specific BAC (bacterial artificial chromosome) clones derived from five reference chromosomes of B. distachyon as probes against the karyotypes of twelve accessions representing five diploid and polyploid Brachypodium perennials. The results showed that descending dysploidy is common in Brachypodium and occurs primarily via nested chromosome fusions. Brachypodium distachyon was rejected as a putative ancestor for allotetraploid perennials and B. stacei for B. mexicanum. We propose two alternative models of perennial polyploid evolution involving either the incorporation of a putative x = 5 ancestral karyotype with di erent descending dysploidy patterns compared to B. distachyon chromosomes or hybridization of two x = 9 ancestors followed by genome doubling and descending dysploidy. Details of the karyotype structure and evolution in several Brachypodium perennials are revealed for the first time.
DOI: 10.3390/ijms20225557
ISSN: 1661-6596
Appears in Collections:Artykuły (WNP)

Files in This Item:
File Description SizeFormat 
Lusinska_Comparatively_barcoded_chromosomes.pdf3,58 MBAdobe PDFView/Open
Show full item record

Uznanie Autorstwa 3.0 Polska Creative Commons License Creative Commons