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Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12128/12594
Title: Synthesis and characterisation of PEG-peptide surfaces for proteolytic enzyme detection
Authors: Trzcińska, Róża
Suder, Piotr
Bodzon-Kułakowska, Anna
Skalska, Magdalena
Marcinkowski, Andrzej
Kubacki, Jerzy
Pedrys, Roman
Silberring, Jerzy
Dworak, Andrzej
Trzebicka, Barbara
Keywords: Interface/surface analysis; Polymers
Issue Date: 2013
Citation: "Analytical and Bioanalytical Chemistry" Vol. 405, iss. 28 (2013), s. 9049-9059
Abstract: Peptide surfaces were obtained by the covalent immobilisation of fluorescently labelled pentapeptides carboxyfluorescein-glycine-arginine–methionine-leucine-glycine, either directly or through a poly(ethylene glycol) (PEG) linker on modified silicon wafers. Each step during the preparation of the peptide surfaces was confirmed by several surface characterisation techniques. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) and X-ray photoelectron spectroscopy were used to determine the surface composition, the wafers philicity was measured by contact angle and atomic force microscopy was used to investigate the surface morphology. Exposure of the peptide surfaces to trypsin resulted in the release of a fluorescently labelled peptide product, which allowed the kinetics of the enzymatic reaction to be followed with the aid of fluorescence spectroscopy.
URI: http://hdl.handle.net/20.500.12128/12594
DOI: 10.1007/s00216-013-7082-z
ISSN: 1618-2642
1618-2650
Appears in Collections:Artykuły (WNŚiT)

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