http://hdl.handle.net/20.500.12128/12594
Tytuł: | Synthesis and characterisation of PEG-peptide surfaces for proteolytic enzyme detection |
Autor: | Trzcińska, Róża Suder, Piotr Bodzoń-Kułakowska, Anna Skalska, Magdalena Marcinkowski, Andrzej Kubacki, Jerzy Pedrys, Roman Silberring, Jerzy Dworak, Andrzej Trzebicka, Barbara |
Słowa kluczowe: | Interface/surface analysis; Polymers |
Data wydania: | 2013 |
Źródło: | "Analytical and Bioanalytical Chemistry" Vol. 405, iss. 28 (2013), s. 9049-9059 |
Abstrakt: | Peptide surfaces were obtained by the covalent immobilisation of fluorescently labelled pentapeptides carboxyfluorescein-glycine-arginine–methionine-leucine-glycine, either directly or through a poly(ethylene glycol) (PEG) linker on modified silicon wafers. Each step during the preparation of the peptide surfaces was confirmed by several surface characterisation techniques. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) and X-ray photoelectron spectroscopy were used to determine the surface composition, the wafers philicity was measured by contact angle and atomic force microscopy was used to investigate the surface morphology. Exposure of the peptide surfaces to trypsin resulted in the release of a fluorescently labelled peptide product, which allowed the kinetics of the enzymatic reaction to be followed with the aid of fluorescence spectroscopy. |
URI: | http://hdl.handle.net/20.500.12128/12594 |
DOI: | 10.1007/s00216-013-7082-z |
ISSN: | 1618-2642 1618-2650 |
Pojawia się w kolekcji: | Artykuły (WNŚiT) |
Plik | Opis | Rozmiar | Format | |
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Trzcinska_Synthesis_and_characterisation_of_PEG.pdf | 843,12 kB | Adobe PDF | Przejrzyj / Otwórz |
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