DC pole | Wartość | Język |
dc.contributor.author | Wójcik, Anna M. | - |
dc.date.accessioned | 2020-07-20T05:59:00Z | - |
dc.date.available | 2020-07-20T05:59:00Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | International Journal of Molecular Sciences, Vol. 21 (2020), art. No. 4969 | pl_PL |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/15205 | - |
dc.description.abstract | During early plant embryogenesis, some of the most fundamental decisions on fate and
identity are taken making it a fascinating process to study. It is no surprise that higher plant
embryogenesis was intensively analysed during the last century, while somatic embryogenesis is
probably the most studied regeneration model. Encoded by the MIRNA, short, single-stranded,
non-coding miRNAs, are commonly present in all Eukaryotic genomes and are involved in the
regulation of the gene expression during the essential developmental processes such as plant
morphogenesis, hormone signaling, and developmental phase transition. During the last few years
dedicated to miRNAs, analytical methods and tools have been developed, which have a orded
new opportunities in functional analyses of plant miRNAs, including (i) databases for in silico
analysis; (ii) miRNAs detection and expression approaches; (iii) reporter and sensor lines for a
spatio-temporal analysis of the miRNA-target interactions; (iv) in situ hybridisation protocols;
(v) artificial miRNAs; (vi) MIM and STTM lines to inhibit miRNA activity, and (vii) the target genes
resistant to miRNA. Here, we attempted to summarise the toolbox for functional analysis of miRNAs
during plant embryogenesis. In addition to characterising the described tools/methods, examples of
the applications have been presented. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | miRNA | pl_PL |
dc.subject | MIRNA genes | pl_PL |
dc.subject | MIM | pl_PL |
dc.subject | STTM | pl_PL |
dc.subject | amiRNA | pl_PL |
dc.subject | in situ hybridisation | pl_PL |
dc.subject | miRNA-resistance | pl_PL |
dc.subject | functional genomics | pl_PL |
dc.subject | embryogenesis | pl_PL |
dc.subject | plant | pl_PL |
dc.title | Research Tools for the Functional Genomics of Plant miRNAs During Zygotic and Somatic Embryogenesis | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.3390/ijms21144969 | - |
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