DC pole | Wartość | Język |
dc.contributor.author | Collin, Anna | - |
dc.contributor.author | Daszkowska-Golec, Agata | - |
dc.contributor.author | Kurowska, Marzena | - |
dc.contributor.author | Szarejko, Iwona | - |
dc.date.accessioned | 2020-08-12T09:54:39Z | - |
dc.date.available | 2020-08-12T09:54:39Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | "Frontiers in Plant Science" Vol. 11 (2020), art. no. 1138, s. 1-23 | pl_PL |
dc.identifier.issn | 1664-462X | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/15575 | - |
dc.description.abstract | ABA INSENSITIVE 5 (ABI5) is a basic leucine zipper (bZIP) transcription factor which acts
in the abscisic acid (ABA) network and is activated in response to abiotic stresses.
However, the precise role of barley (Hordeum vulgare) ABI5 in ABA signaling and its
function under stress remains elusive. Here, we show that HvABI5 is involved in ABAdependent
regulation of barley response to drought stress. We identified barley TILLING
mutants carrying different alleles in the HvABI5 gene and we studied in detail the
physiological and molecular response to drought and ABA for one of them. The
hvabi5.d mutant, carrying G1751A transition, was insensitive to ABA during seed
germination, yet it showed the ability to store more water than its parent cv.
“Sebastian” (WT) in response to drought stress. The drought-tolerant phenotype of
hvabi5.d was associated with better membrane protection, higher flavonoid content,
and faster stomatal closure in the mutant under stress compared to the WT. The
microarray transcriptome analysis revealed up-regulation of genes associated with cell
protection mechanisms in the mutant. Furthermore, HvABI5 target genes: HVA1 and
HVA22 showed higher activity after drought, which may imply better adaptation of
hvabi5.d to stress. On the other hand, chlorophyll content in hvabi5.d was lower than
in WT, which was associated with decreased photosynthesis efficiency observed in the
mutant after drought treatment. To verify that HvABI5 acts in the ABA-dependent manner
we analyzed expression of selected genes related to ABA pathway in hvabi5.d and its WT
parent after drought and ABA treatments. The expression of key genes involved in ABA
metabolism and signaling differed in the mutant and the WT under stress. Droughtinduced
increase of expression of HvNCED1, HvBG8, HvSnRK2.1, and HvPP2C4 genes
was 2–20 times higher in hvabi5.d compared to “Sebastian”. We also observed a faster
stomatal closure in hvabi5.d and much higher induction of HvNCED1 and HvSnRK2.1
genes after ABA treatment. Together, these findings demonstrate that HvABI5 plays a role
in regulation of drought response in barley and suggest that HvABI5 might be engaged in
the fine tuning of ABA signaling by a feedback regulation between biosynthetic and
signaling events. In addition, they point to different mechanisms of HvABI5 action in
regulating drought response and seed germination in barley. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | abscisic acid | pl_PL |
dc.subject | monocots | pl_PL |
dc.subject | barley | pl_PL |
dc.subject | Hordeum vulgare | pl_PL |
dc.subject | water deficit | pl_PL |
dc.subject | stress | pl_PL |
dc.subject | transcriptomics | pl_PL |
dc.subject | ABI5 | pl_PL |
dc.title | Barley ABI5 (Abscisic Acid INSENSITIVE 5) Is Involved in Abscisic Acid-Dependent Drought Response | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.3389/fpls.2020.01138 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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