DC pole | Wartość | Język |
dc.contributor.author | Żur, Joanna | - |
dc.contributor.author | Piński, Artur | - |
dc.contributor.author | Wojcieszyńska, Danuta | - |
dc.contributor.author | Smułek, Wojciech | - |
dc.contributor.author | Guzik, Urszula | - |
dc.date.accessioned | 2020-09-17T09:54:23Z | - |
dc.date.available | 2020-09-17T09:54:23Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | International Journal of Molecular Sciences, Vol. 21, iss. 18 (2020) art. no. 6786 | pl_PL |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/15970 | - |
dc.description.abstract | Diclofenac (DCF) constitutes one of the most significant ecopollutants detected in various
environmental matrices. Biological clean-up technologies that rely on xenobiotics-degrading
microorganisms are considered as a valuable alternative for chemical oxidation methods. Up to
now, the knowledge about DCF multi-level influence on bacterial cells is fragmentary. In this
study, we evaluate the degradation potential and impact of DCF on Pseudomonas moorei KB4 strain.
In mono-substrate culture KB4 metabolized 0.5 mg L-1 of DCF, but supplementation with glucose
(Glc) and sodium acetate (SA) increased degraded doses up to 1 mg L-1 within 12 days. For all
established conditions, 4'-OH-DCF and DCF-lactam were identified. Gene expression analysis
revealed the up-regulation of selected genes encoding biotransformation enzymes in the presence of
DCF, in both mono-substrate and co-metabolic conditions. The multifactorial analysis of KB4 cell
exposure to DCF showed a decrease in the zeta-potential with a simultaneous increase in the cell wall
hydrophobicity. Magnified membrane permeability was coupled with the significant increase in the
branched (19:0 anteiso) and cyclopropane (17:0 cyclo) fatty acid accompanied with reduced amounts
of unsaturated ones. DCF injures the cells which is expressed by raised activities of acid and alkaline
phosphatases as well as formation of lipids peroxidation products (LPX). The elevated activity of
superoxide dismutase (SOD) and catalase (CAT) testified that DCF induced oxidative stress. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | biotransformation enzymes | pl_PL |
dc.subject | cells injury | pl_PL |
dc.subject | diclofenac | pl_PL |
dc.subject | gene expression | pl_PL |
dc.subject | membranes | pl_PL |
dc.subject | metabolites | pl_PL |
dc.subject | oxidative stress | pl_PL |
dc.subject | Pseudomonas | pl_PL |
dc.subject | toxicity | pl_PL |
dc.title | Diclofenac degradation - enzymes, genetic background and cellular alterations triggered in diclofenac-metabolizing strain Pseudomonas moorei KB4 | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.3390/ijms21186786 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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