http://hdl.handle.net/20.500.12128/16047
Title: | Modulation of FAD-dependent monooxygenase activity from aromatic compounds-degrading Stenotrophomonas maltophilia strain KB2 |
Authors: | Wojcieszyńska, Danuta Greń, Izabela Hupert-Kocurek, Katarzyna Guzik, Urszula |
Keywords: | monooxygenase; Stenotrophomonas; phenols; inhibitors |
Issue Date: | 2011 |
Citation: | Acta Biochimica Polonica, 2011, no. 3, s. 421-426 |
Abstract: | The purpose of this study was purification and charac-terization of phenol monooxygenase from Stenotropho-monas maltophilia strain KB2, enzyme that catabolises phenol and its derivatives through the initial hydroxyla-tion to catechols. The enzyme requires NADH and FAD as a cofactors for activity, catalyses hydroxylation of a wide range of monocyclic phenols, aromatic acids and dihydroxylated derivatives of benzene except for cate-chol. High activity of this monooxygenase was observed in cell extract of strain KB2 grown on phenol, 2-meth-ylphenol, 3-metylphenol or 4-methylphenol. Ionic sur-factants as well as cytochrome P450 inhibitors or 1,4-di-oxane, acetone and n-butyl acetate inhibited the en-zyme activity, while non-ionic surfactants, chloroethane, ethylbenzene, ethyl acetate, cyclohexane, and benzene enhanced it. These results indicate that the phenol mo-nooxygenase from Stenotrophomonas maltophilia strain KB2 holds great potential for bioremediation. |
URI: | http://hdl.handle.net/20.500.12128/16047 |
ISSN: | 1734-154X 0001-527X |
Appears in Collections: | Artykuły (WNP) |
File | Description | Size | Format | |
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Wojcieszynska_Modulatio_of_FAD_dependent_monooxygenase.pdf | 583,11 kB | Adobe PDF | View/Open |
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