http://hdl.handle.net/20.500.12128/16054
Tytuł: | Hypermethylation of Auxin-Responsive Motifs in the Promoters of the Transcription Factor Genes Accompanies the Somatic Embryogenesis Induction in Arabidopsis |
Autor: | Grzybkowska, Daria Nowak, Katarzyna Gaj, Małgorzata D. |
Słowa kluczowe: | DNA methylation; somatic embryogenesis; SE-related genes |
Data wydania: | 2020 |
Źródło: | International Journal of Molecular Sciences, Vol. 21 (2020), Art. No. 6849 |
Abstrakt: | The auxin-induced embryogenic reprogramming of plant somatic cells is associated with extensive modulation of the gene expression in which epigenetic modifications, including DNA methylation, seem to play a crucial role. However, the function of DNA methylation, including the role of auxin in epigenetic regulation of the SE-controlling genes, remains poorly understood. Hence, in the present study, we analysed the expression and methylation of the TF genes that play a critical regulatory role during SE induction (LEC1, LEC2, BBM, WUS and AGL15) in auxin-treated explants of Arabidopsis. The results showed that auxin treatment substantially a ected both the expression and methylation patterns of the SE-involved TF genes in a concentration-dependent manner. The auxin treatment di erentially modulated the methylation of the promoter (P) and gene body (GB) sequences of the SE-involved genes. Relevantly, the SE-e ective auxin treatment (5.0 M of 2,4-D) was associated with the stable hypermethylation of the P regions of the SE-involved genes and a significantly higher methylation of the P than the GB fragments was a characteristic feature of the embryogenic culture. The presence of auxin-responsive (AuxRE) motifs in the hypermethylated P regions suggests that auxin might substantially contribute to the DNA methylation-mediated control of the SE-involved genes. |
URI: | http://hdl.handle.net/20.500.12128/16054 |
DOI: | 10.3390/ijms21186849 |
ISSN: | 1661-6596 1422-0067 |
Pojawia się w kolekcji: | Artykuły (WNP) |
Plik | Opis | Rozmiar | Format | |
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Grzybkowska_Hypermethylation_of_Auxin-Responsive_motifs.pdf | 2,42 MB | Adobe PDF | Przejrzyj / Otwórz |
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