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Tytuł: Immobilization as a Strategy for Improving Enzyme Properties-Application to Oxidoreductases
Autor: Guzik, Urszula
Hupert-Kocurek, Katarzyna
Wojcieszyńska, Danuta
Słowa kluczowe: oxidoreductases; immobilization; stability of enzyme
Data wydania: 2014
Źródło: Molecules, (2014), iss. 7, s. 8995-9018
Abstrakt: The main objective of the immobilization of enzymes is to enhance the economics of biocatalytic processes. Immobilization allows one to re-use the enzyme for an extended period of time and enables easier separation of the catalyst from the product. Additionally, immobilization improves many properties of enzymes such as performance in organic solvents, pH tolerance, heat stability or the functional stability. Increasing the structural rigidity of the protein and stabilization of multimeric enzymes which prevents dissociation-related inactivation. In the last decade, several papers about immobilization methods have been published. In our work, we present a relation between the influence of immobilization on the improvement of the properties of selected oxidoreductases and their commercial value. We also present our view on the role that different immobilization methods play in the reduction of enzyme inhibition during biotechnological processes.
DOI: 10.3390/molecules19078995
ISSN: 1420-3049
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