Skip navigation

Please use this identifier to cite or link to this item:
Title: DNA Demethylation in Response to Heat Stress in Arabidopsis thaliana
Authors: Korotko, Urszula
Chwiałkowska, Karolina
Sańko-Sawczenko, Izabela
Kwaśniewski, Mirosław
Keywords: abiotic stress; epigenetics; heat shock proteins
Issue Date: 2021
Citation: Molecular Sciences, 2021, Vol. 22, art. no. 1555
Abstract: Environmental stress is one of the most important factors affecting plant growth and development. Recent studies have shown that epigenetic mechanisms, such as DNA methylation, play a key role in adapting plants to stress conditions. Here, we analyzed the dynamics of changes in the level of DNA methylation in Arabidopsis thaliana (L.) Heynh. (Brassicaceae) under the influence of heat stress. For this purpose, whole-genome sequencing of sodium bisulfite-treated DNA was performed. The analysis was performed at seven time points, taking into account the control conditions, heat stress, and recovery to control conditions after the stress treatment was discontinued. In our study we observed decrease in the level of DNA methylation under the influence of heat stress, especially after returning to control conditions. Analysis of the gene ontology enrichment and regulatory pathways showed that genes characterized by differential DNA methylation are mainly associated with stress response, including heat stress. These are the genes encoding heat shock proteins and genes associated with translation regulation. A decrease in the level of DNA methylation in such specific sites suggests that under the influence of heat stress we observe active demethylation phenomenon rather than passive demethylation, which is not locus specific.
DOI: 10.3390/ijms22041555
ISSN: 1422-0067
Appears in Collections:Artykuły (WNP)

Files in This Item:
File Description SizeFormat 
Korotko_DNA_Demethylation_in_Response.pdf2,69 MBAdobe PDFView/Open
Show full item record

Uznanie Autorstwa 3.0 Polska Creative Commons License Creative Commons