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dc.contributor.authorGodel-Jędrychowska, Kamila-
dc.contributor.authorSala, Katarzyna-
dc.contributor.authorMilewska-Hendel, Anna-
dc.contributor.authorZubko, Maciej-
dc.contributor.authorKurczyńska, Ewa-
dc.contributor.authorOleszkiewicz, Tomasz-
dc.contributor.authorKlimek-Chodacka, Magdalena-
dc.contributor.authorKruczek, Michał-
dc.contributor.authorQi, Yiping-
dc.contributor.authorBarański, Rafał-
dc.identifier.citation"International Journal of Molecular Sciences" (2021), iss. 12, art. no. 6516, s. 1-23pl_PL
dc.description.abstractRecent data indicate that modifications to carotenoid biosynthesis pathway in plants alter the expression of genes affecting chemical composition of the cell wall. Phytoene synthase (PSY) is a rate limiting factor of carotenoid biosynthesis and it may exhibit species-specific and organ-specific roles determined by the presence of psy paralogous genes, the importance of which often remains unrevealed. Thus, the aim of this work was to elaborate the roles of two psy paralogs in a model system and to reveal biochemical changes in the cell wall of psy knockout mutants. For this purpose, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR associated (Cas9) proteins (CRISPR/Cas9) vectors were introduced to carotenoid-rich carrot (Daucus carota) callus cells in order to induce mutations in the psy1 and psy2 genes. Gene sequencing, expression analysis, and carotenoid content analysis revealed that the psy2 gene is critical for carotenoid biosynthesis in this model and its knockout blocks carotenogenesis. The psy2 knockout also decreased the expression of the psy1 paralog. Immunohistochemical staining of the psy2 mutant cells showed altered composition of arabinogalactan proteins, pectins, and extensins in the mutant cell walls. In particular, low-methylesterified pectins were abundantly present in the cell walls of carotenoidrich callus in contrast to the carotenoid-free psy2 mutant. Transmission electron microscopy revealed altered plastid transition to amyloplasts instead of chromoplasts. The results demonstrate for the first time that the inhibited biosynthesis of carotenoids triggers the cell wall remodelling.pl_PL
dc.rightsUznanie autorstwa 3.0 Polska*
dc.subjectarabinogalactan proteinpl_PL
dc.subjectCas9 proteinpl_PL
dc.subjectClustered Regularly Interspaced Short Palindromic Repeatspl_PL
dc.subjectphytoene synthasepl_PL
dc.subjectplastid ultrastructurepl_PL
dc.titleInhibition of carotenoid biosynthesis by CRISPR/Cas9triggers cell wall remodelling in carrotpl_PL
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