Skip navigation

Please use this identifier to cite or link to this item:
Title: Non-Targeted Metabolite Profiling Reveals Host Metabolomic Reprogramming during the Interaction of Black Pepper with Phytophthora capsici
Authors: Kattupalli, Divya
Piński, Artur
Sreekumar, Swea
Usha, Aswathi
Giryjia, Aiswarya
Beckmann, Manfred
Mur, Luis Alejandro Jose
Vasudevan, Soniya Eppurathu
Keywords: Piper nigrum; quick wilt; metabolomics; abscisic acid; salicylic acid; jasmonic acid
Issue Date: 2021
Citation: "International Journal of Molecular Science" (2021), iss. 21, art. no. 11433, s. 1-17
Abstract: Phytophthora capsici is one of the most destructive pathogens causing quick wilt (foot rot) disease in black pepper (Piper nigrum L.) to which no effective resistance has been defined. To better understand the P. nigrum–P. capsici pathosystem, we employed metabolomic approaches based on flow‐infusion electrospray–high‐resolution mass spectrometry. Changes in the leaf metabolome were assessed in infected and systemic tissues at 24 and 48 hpi. Principal Component Analysis of the derived data indicated that the infected leaves showed a rapid metabolic response by 24 hpi whereas the systemic leaves took 48 hpi to respond to the infection. The major sources of variations between infected leaf and systemic leaf were identified, and enrichment pathway analysis indicated, major shifts in amino acid, tricarboxylic acid cycle, nucleotide and vitamin B6 metabolism upon infection. Moreover, the individual metabolites involved in defensive phytohormone signalling were identified. RT‐qPCR analysis of key salicylate and jasmonate biosynthetic genes indicated a transient reduction of expression at 24 hpi but this increased subsequently. Exogenous application of jasmonate and salicylate reduced P. capsici disease symptoms, but this effect was suppressed with the co‐application of abscisic acid. The results are consistent with abscisic acid reprogramming, salicylate and jasmonate defences in infected leaves to facilitate the formation of disease. The augmentation of salicylate and jasmonate defences could represent an approach through which quick wilt disease could be controlled in black pepper.
DOI: 10.3390/ijms222111433
ISSN: 1422-0067
Appears in Collections:Artykuły (WNP)

Files in This Item:
File Description SizeFormat 
Pinski_non_targeted_metabolite_profiling.pdf1,87 MBAdobe PDFView/Open
Show full item record

Uznanie Autorstwa 3.0 Polska Creative Commons License Creative Commons