DC pole | Wartość | Język |
dc.contributor.author | Morończyk, Joanna | - |
dc.contributor.author | Brąszewska, Agnieszka | - |
dc.contributor.author | Wójcikowska, Barbara | - |
dc.contributor.author | Chwiałkowska, Karolina | - |
dc.contributor.author | Nowak, Katarzyna | - |
dc.contributor.author | Wójcik, Anna M. | - |
dc.contributor.author | Kwaśniewski, Mirosław | - |
dc.contributor.author | Gaj, Małgorzata D. | - |
dc.date.accessioned | 2022-03-08T13:13:29Z | - |
dc.date.available | 2022-03-08T13:13:29Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | "Cells" (2022), vol. 11, nr 5, art. no. 863 | pl_PL |
dc.identifier.issn | 2073-4409 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/22791 | - |
dc.description.abstract | Somatic embryogenesis (SE), which is a process that involves the in vitro-induced embryogenic
reprogramming of plant somatic cells, requires dynamic changes in the cell transcriptome. These
changes are fine-tuned by many genetic and epigenetic factors, including posttranslational histone
modifications such as histone acetylation. Antagonistically acting enzymes, histone acetyltransferases
(HATs) and deacetylases (HDACs), which control histone acetylation in many developmental processes,
are believed to control SE. However, the function of specific HAT/HDACs and the genes
that are subjected to histone acetylation-mediated regulation during SE have yet to be revealed.
Here, we present the global and gene-specific changes in histone acetylation in Arabidopsis explants
that are undergoing SE. In the TSA (trichostatin A)-induced SE, we demonstrate that H3 and H4
acetylation might control the expression of the critical transcription factor (TF) genes of a vital role in
SE, including LEC1, LEC2 (LEAFY COTYLEDON 1; 2), FUS3 (FUSCA 3) and MYB118 (MYB DOMAIN
PROTEIN 118). Within the HATs and HDACs, which mainly positively regulate SE, we identified
HDA19 as negatively affecting SE by regulating LEC1, LEC2 and BBM. Finally, we provide some
evidence on the role of HDA19 in the histone acetylation-mediated regulation of LEC2 during SE. Our
results reveal an essential function of histone acetylation in the epigenetic mechanisms that control
the TF genes that play critical roles in the embryogenic reprogramming of plant somatic cells. The
results implicate the complexity of Hac-related gene regulation in embryogenic induction and point
to differences in the regulatory mechanisms that are involved in auxin- and TSA-induced SE. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | Arabidopsis | pl_PL |
dc.subject | auxin | pl_PL |
dc.subject | histone acetylation | pl_PL |
dc.subject | somatic embryogenesis | pl_PL |
dc.subject | transcription factors | pl_PL |
dc.subject | trichostatin A | pl_PL |
dc.title | Insights into the Histone Acetylation-Mediated Regulation of the Transcription Factor Genes That Control the Embryogenic Transition in the Somatic Cells of Arabidopsis | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.3390/cells11050863 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
|