Pressure-induced cell wall instability and growth oscillations in pollen tubes

Abstract

In the seed plants, the pollen tube is a cellular extension that serves as a conduit through which male gametes are transported to complete fertilization of the egg cell. It consists of a single elongated cell which exhibits characteristic oscillations in growth rate until it finally bursts, completing its function. The mechanism behind the periodic character of the growth has not been fully understood. In this paper we show that the mechanism of pressure - induced symmetry frustration occurring in the wall at the transition-perimeter between the cylindrical and approximately hemispherical parts of the growing pollen tube, together with the addition of cell wall material, is sufficient to release and sustain mechanical self-oscillations and cell extension. At the transition zone, where symmetry frustration occurs and one cannot distinguish either of the involved symmetries, a kind of 'superposition state' appears where either single or both symmetry(ies) can be realized by the system. We anticipate that testifiable predictions made by the model (f ∝ √P) may deliver, after calibration, a new tool to estimate turgor pressure P from oscillation frequency f of the periodically growing cell. Since the mechanical principles apply to all turgor regulated walled cells including those of plant, fungal and bacterial origin, the relevance of this work is not limited to the case of the pollen tube.