DC pole | Wartość | Język |
dc.contributor.author | Cycoń, Mariusz | - |
dc.contributor.author | Borymski, Sławomir | - |
dc.contributor.author | Orlewska, Kamila | - |
dc.contributor.author | Wąsik, Tomasz J. | - |
dc.contributor.author | Piotrowska-Seget, Zofia | - |
dc.date.accessioned | 2018-04-23T05:57:54Z | - |
dc.date.available | 2018-04-23T05:57:54Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Frontiers in Microbiology, Vol. 7 (2016), art. no. 1015) | pl_PL |
dc.identifier.issn | 1664-302X | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/3008 | - |
dc.description.abstract | The occurrence of antibiotics and antibiotic resistance genes in the environment has become a subject of growing concern. The extensive use of vancomycin and other pharmaceuticals may alter the biodiversity of soil microbial communities and select antibiotic-resistant bacteria. Therefore, the purpose of the study was to evaluate the impact of vancomycin and/or vancomycin-resistant Citrobacter freundii on soil microbial communities using the denaturing gradient gel electrophoresis (DGGE) and the phospholipid fatty acid (PLFA) approaches. The experiment had a completely randomized block design with the following treatments: Control soil (C), soil with vancomycin (1 mg/kg soil-VA1), soil with vancomycin (10 mg/kg soil-VA10), soil with C. freundii (Cit), soil with vancomycin (1 mg/kg soil) and C. freundii (VA1+Cit), and soil with vancomycin (10 mg/kg soil) and C. freundii (VA10+Cit). A bacterial strain resistant to vancomycin was isolated from raw sewage collected from the municipal sewage treatment plant. The obtained results indicated that the antibiotic and/or the bacterial strain exerted a selective pressure that resulted in qualitative and quantitative changes in the population of soil microorganisms. However, a multivariate analysis showed that the genetic and structural diversity of the soil microbial community was primarily affected by the incubation time and to a lesser extent by the antibiotic and introduced bacteria. DGGE analysis clearly showed that certain species within the bacterial community were sensitive to vancomycin as was evidenced by a decrease in the values of S (richness) and H (Shannon-Wiener) indices. Moreover, a PLFA method-based analysis revealed alterations in the structure of the soil microbial community as indicated by changes in the biomass of the PLFA biomarkers specific for Gram-positive and Gram-negative bacteria as well as fungi. The changes observed in the community of soil microorganisms may decrease the rate of microbial-mediated processes, which can lead to a disturbance in the ecological balance of the soil ecosystem. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | Antibiotic resistance | pl_PL |
dc.subject | Citrobacter freundii | pl_PL |
dc.subject | Vancomycin | pl_PL |
dc.subject | Soil | pl_PL |
dc.title | An analysis of the effects of vancomycin and/or vancomycin-resistant Citrobacter freundii exposure on the microbial community structure in soil | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.3389/fmicb.2016.01015 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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