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Title: Histone H3 and H4 acetylation patterns are more dynamic than those of DNA methylation in Brachypodium distachyon embryos during seed maturation and germination
Authors: Wolny, Elżbieta
Brąszewska-Zalewska, Agnieszka
Kroczek, Daria
Hasterok, Robert
Keywords: Brachypodium distachyon embryo; DNA methylation; Epigenetic modifications; H3K18ac; H4K16ac
Issue Date: 2017
Citation: Protoplasma, vol. 254, iss. 5 (2017), s. 2045-2052
Abstract: The transition of seeds from a dry to a metabolically active state requires significant changes in both the spatial and temporal patterns of gene expression, and this transcriptional reprogramming involves various modifications of the chromatin structure. There are several factors that can greatly influence the structure of chromatin, one of which is the chemical modifications of histone proteins and DNA itself. In this study, we analysed the distribution of three epigenetic markers, i.e. acetylation of histone H4 (H4K16ac) and histone H3 (H3K18ac) as well as DNA methylation (5mC) in Brachypodium distachyon embryos during the four stages of seed development—maturation, desiccation (quiescence), imbibition and germination. Our results indicate that both H4K16ac and H3K18ac are at a very high level in embryos during seed imbibition, but that the patterns of DNA methylation are considerably more stable in embryos during seed development.
DOI: 10.1007/s00709-017-1088-x
ISSN: 0033-183X
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