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Title: Frequently asked questions about chlorophyll fluorescence, the sequel
Authors: Kalaji, Hazem M.
Schansker, Gert
Brestic, Marian
Bussotti, Filippo
Calatayud, Angeles
Ferroni, Lorenzo
Goltsev, Vasilij
Guidi, Lucia
Jajoo, Anjana
Li, Pengmin
Losciale, Pasquale
Mishra, Vinod K.
Misra, Amarendra N.
Nebauer, Sergio G.
Pancaldi, Simonetta
Penella, Consuelo
Pollastrini, Martina
Suresh, Kancherla
Tambussi, Eduardo
Yanniccari, Marcos
Zivcak, Marek
Cetner, Magdalena D.
Samborska, Izabela A.
Stirbet, Alexandrina
Olsovska, Katarina
Kunderlikova, Kristyna
Shelonzek, Henry
Rusinowski, Szymon
Bąba, Wojciech
Keywords: Area; Chl a fluorescence; Delayed fluorescence; Energy partitioning; Photochemical quenching
Issue Date: 2017
Citation: Photosynthesis Research, vol. 132, iss. 1 (2017), s. 13-66
Abstract: Using chlorophyll (Chl) a fluorescence many aspects of the photosynthetic apparatus can be studied, both in vitro and, noninvasively, in vivo. Complementary techniques can help to interpret changes in the Chl a fluorescence kinetics. Kalaji et al. (Photosynth Res 122:121–158, 2014a) addressed several questions about instruments, methods and applications based on Chl a fluorescence. Here, additional Chl a fluorescence-related topics are discussed again in a question and answer format. Examples are the effect of connectivity on photochemical quenching, the correction of FV/FM values for PSI fluorescence, the energy partitioning concept, the interpretation of the complementary area, probing the donor side of PSII, the assignment of bands of 77 K fluorescence emission spectra to fluorescence emitters, the relationship between prompt and delayed fluorescence, potential problems when sampling tree canopies, the use of fluorescence parameters in QTL studies, the use of Chl a fluorescence in biosensor applications and the application of neural network approaches for the analysis of fluorescence measurements. The answers draw on knowledge from different Chl a fluorescence analysis domains, yielding in several cases new insights.
DOI: 10.1007/s11120-016-0318-y
ISSN: 0166-8595
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