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Title: Role of auxin (IAA) in the regulation of slow vacuolar (SV) channels and the volume of red beet taproot vacuoles
Authors: Burdach, Zbigniew
Siemieniuk, Agnieszka
Trela, Zenon
Kurtyka, Renata
Karcz, Waldemar
Keywords: Beta vulgaris L.; IAA (indole-3-acetic acid); SV channels; Vacuolar volume; Vacuole
Issue Date: 2018
Citation: BMC Plant Biology, Vol. 18, iss. 1 (2018), art. no. 102
Abstract: Background: Auxin (IAA) is a central player in plant cell growth. In contrast to the well-established function of the plasma membrane in plant cell expansion, little is known about the role of the vacuolar membrane (tonoplast) in this process. Results: It was found that under symmetrical 100 mM K+ and 100 μM cytoplasmic Ca2+ the macroscopic currents showed a typical slow activation and a strong outward rectification of the steady-state currents. The addition of IAA at a final concentration of 1 μM to the bath medium stimulated the SV currents, whereas at 0.1 and 10 μM slight inhibition of SV currents was observed. The time constant, decreased in the presence of this hormone. When single channels were analyzed, an increase in their activity was recorded with IAA compared to the control. The single-channel recordings that were obtained in the presence of IAA showed that auxin increased the amplitude of the single-channel currents. Interestingly, the addition of IAA to the bath medium with the same composition as the one that was used in the patch-clamp experiments showed that auxin decreased the volume of the vacuoles. Conclusions: It is suggested that the SV channels and the volume of red beet taproot vacuoles are modulated by auxin (IAA).
DOI: 10.1186/s12870-018-1321-6
ISSN: 1471-2229
Appears in Collections:Artykuły (WNP)

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