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Title: Development of microsatellite markers using pyrosequencing in Galium trifidum (Rubiaceae), a rare species in central Europe
Authors: Szczecińska, Monika
Kwaśniewski, Mirosław
Sawicki, Jakub
Chwiałkowska, Karolina
Szandar, Kamil
Pisarek, Włodzimierz
Keywords: 454 pyrosequencing; Galium trifidum; SSR markers; Genetic Markers; Rubiaceae
Issue Date: 2012
Citation: International Journal of Molecular Sciences, (2012), iss. 8, p. 9893-9899
Abstract: We identify a large number of microsatellites from Galium trfidum, a plant species considered rare and endangered in Central and Western Europe. Using a combination of a total enriched genomic library and small-scale 454 pyrosequencing, we determined 9755 contigs with a length of 100 to 6192 bp. Within this dataset, we identified 153 SSR motifs in 144 contigs. Here, we tested 14 microsatellite loci in 2 populations of G. trifidum. The number of alleles and expected heterozygosity were 1-8 (mean 3.2) and 0.00-0.876 (0.549 on average), respectively. The markers described in this study will be useful for evaluating genetic diversity within and between populations, and gene flow between G. trifidum populations. These markers could also be applied to investigate the biological aspects of G. trifidum, such as the population dynamics and clonal structure, and to develop effective conservation programs for the Central European opulations of this species.
DOI: 10.3390/ijms13089893
ISSN: 1661-6596
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