DC pole | Wartość | Język |
dc.contributor.author | Wójcik, Anna M. | - |
dc.contributor.author | Mosiolek, Magdalena | - |
dc.contributor.author | Karcz, Jagna | - |
dc.contributor.author | Nodine, Michael D. | - |
dc.contributor.author | Gaj, Małgorzata D. | - |
dc.date.accessioned | 2018-09-14T11:00:46Z | - |
dc.date.available | 2018-09-14T11:00:46Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Frontiers in Plant Science, 4 Sept. 2018 | pl_PL |
dc.identifier.issn | 1664-462X | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/6194 | - |
dc.description.abstract | Somatic embryogenesis (SE) results from the transition of differentiated plant somatic
cells into embryogenic cells that requires the extensive reprogramming of the somatic
cell transcriptome. Commonly, the SE-involved genes are identified by analyzing the
heterogeneous population of explant cells and thus, it is necessary to validate the
expression of the candidate genes in the cells that are competent for embryogenic
transition. Here, we optimized and implemented the whole mount in situ hybridization
(WISH) method (Bleckmann and Dresselhaus, 2016; Dastidar et al., 2016) in order to
analyze the spatiotemporal localization of miRNAs (miR156, miR166, miR390, miR167)
and mRNAs such as WOX5 and PHABULOSA-target of miR165/166 during the SE
that is induced in Arabidopsis explants. This study presents a detailed step-by-step
description of the WISH procedure in which DIG-labeled LNA and RNA probes were
used to detect miRNAs and mRNAs, respectively. The usefulness of the WISH in the
functional analysis of the SE-involved regulatory pathways is demonstrated and the
advantages of this method are highlighted: (i) the ability to analyze intact non-sectioned
plant tissue; (ii) the specificity of transcript detection; (iii) the detection of miRNA; and (iv)
a semi-quantitative assessment of the RNA abundance. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | WISH | pl_PL |
dc.subject | miRNA | pl_PL |
dc.subject | mRNA | pl_PL |
dc.subject | somatic embryogenesis | pl_PL |
dc.subject | Arabidopsis | pl_PL |
dc.subject | in situ hybridisation | pl_PL |
dc.subject | in vitro | pl_PL |
dc.title | Whole Mount in situ Localization of miRNAs and mRNAs During Somatic Embryogenesis in Arabidopsis | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.3389/fpls.2018.01277 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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