DC pole | Wartość | Język |
dc.contributor.author | Gajewska, Patrycja | - |
dc.contributor.author | Janiak, Agnieszka | - |
dc.contributor.author | Kwaśniewski, Mirosław | - |
dc.contributor.author | Kędziorski, Piotr | - |
dc.contributor.author | Szarejko, Iwona | - |
dc.date.accessioned | 2018-09-14T11:00:53Z | - |
dc.date.available | 2018-09-14T11:00:53Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Frontiers in Plant Science, 3 Sept. 2018 | pl_PL |
dc.identifier.issn | 1664-462X | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/6195 | - |
dc.description.abstract | Root hairs are the part of root architecture contributing significantly to the root surface
area. Their role is particularly substantial in maintaining plant growth under stress
conditions, however, knowledge on mechanism of root hair differentiation is still limited
for majority of crop species, including barley. Here, we report the results of a mapbased
identification of a candidate gene responsible for the lack of root epidermal cell
differentiation, which results in the lack of root hairs in barley. The analysis was based
on the root hairless barley mutant rhl1.b, obtained after chemical mutagenesis of spring
cultivar ‘Karat’. The rhl1 gene was located in chromosome 7HS in our previous studies.
Fine mapping allowed to narrow the interval encompassing rhl1 gene to 3.7 cM, which
on physical barley map spans a region of 577 kb. Five high confidence genes are located
within this region and their sequencing resulted in the identification of A>T mutation
in one candidate, HORVU7Hr1G030250 (MLOC_38567), differing the mutant from its
parent variety. The mutation, located in the 30 splice-junction site, caused the retention
of the last intron, 98 bp long, in mRNA of rhl1.b allele. This resulted in the frameshift, the
synthesis of 71 abnormal amino acids and introduction of premature STOP codon in
mRNA. The mutation was present in the recombinants from the mapping population
(F2 rhl1.b ‘Morex’) that lacked root hairs. The candidate gene encodes a bHLH
transcription factor with LRL domain and may be involved in early stages of root hair
cell development. We discuss the possible involvement of HORVU7Hr1G030250 in this
process, as the best candidate responsible for early stages of rhizodermis differentiation
in barley. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | barley | pl_PL |
dc.subject | gene mapping | pl_PL |
dc.subject | mutants | pl_PL |
dc.subject | root hairs | pl_PL |
dc.subject | rhizodermis pattern | pl_PL |
dc.title | Forward Genetics Approach Reveals a Mutation in bHLH Transcription Factor-Encoding Gene as the Best Candidate for the Root Hairless Phenotype in Barley | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.3389/fpls.2018.01229 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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