DC pole | Wartość | Język |
dc.contributor.author | Betekhtin, Alexander | - |
dc.contributor.author | Rojek, Magdalena | - |
dc.contributor.author | Nowak, Katarzyna | - |
dc.contributor.author | Piński, Artur | - |
dc.contributor.author | Milewska-Hendel, Anna | - |
dc.contributor.author | Kurczyńska, Ewa | - |
dc.contributor.author | Doonan, John H. | - |
dc.contributor.author | Hasterok, Robert | - |
dc.date.accessioned | 2018-11-30T09:16:50Z | - |
dc.date.available | 2018-11-30T09:16:50Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | International Journal of Molecular Sciences, Vol. 19, iss. 12 (2018), Art. No. 3811 | pl_PL |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/7296 | - |
dc.description.abstract | Effective regeneration of callus tissue into embryos and then into whole plants is essential
for plant biotechnology. The embryonic potential is often low and can further decrease with
time in culture, which limits the utilisation of calli for transformation procedures and in vitro
propagation. In this study, we show that the loss of embryogenic potential in callus cultures of
Brachypodium distachyon is progressive over time. Flow cytometry analyses indicated endoploidy
levels increased in 60- and 90-day-old calli with effective loss of the 2C DNA content peak in
the latter. Analysis of indolic compounds content revealed a decrease in 60- and 90-day-old calli
compared to either freshly isolated explants or 30-day-old calli. Immunohistochemical analysis
revealed a decrease in arabinogalactan proteins (AGP) signal with the time of culture, but extensin
(EXT) epitopes either increased (JIM12 epitopes) or decreased (JIM11 epitopes). The transcript
accumulation levels of AGPs and EXTs confirmed these results, with most of AGP and EXT transcripts
gradually decreasing. Some chimeric EXT transcripts significantly increased on the 30th day of culture,
perhaps because of an increased embryogenic potential. Selected somatic embryogenesis-related
genes and cyclins demonstrated a gradual decrease of transcript accumulation for YUCCA (YUC),
AINTEGUMENTA-LIKE (AIL), BABY BOOM (BBM), and CLAVATA (CLV3) genes, as well as for most
of the cyclins, starting from the 30th day of culture. Notably, WUSCHEL (WUS) transcript was
detectable only on the 30th and 60th day and was not detectable in the zygotic embryos and in
90-day-old calli. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | arabinogalactan proteins | pl_PL |
dc.subject | Brachypodium distachyon | pl_PL |
dc.subject | cell wall | pl_PL |
dc.subject | cyclins | pl_PL |
dc.subject | extensins | pl_PL |
dc.subject | pectins | pl_PL |
dc.subject | ploidy instability | pl_PL |
dc.subject | somatic embryogenesis | pl_PL |
dc.subject | somaclonal variation | pl_PL |
dc.subject | transcript accumulation | pl_PL |
dc.title | Cell Wall Epitopes and Endoploidy as Reporters of Embryogenic Potential in Brachypodium Distachyon Callus Culture | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.relation.journal | International Journal of Molecular Sciences | pl_PL |
dc.identifier.doi | 10.3390/ijms19123811 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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