Skip navigation

Please use this identifier to cite or link to this item:
Title: Plant Species and Heavy Metals Affect Biodiversity of Microbial Communities Associated With Metal-Tolerant Plants in Metalliferous Soils
Authors: Borymski, Sławomir
Cycoń, Mariusz
Beckmann, Manfred
Mur, Luis A. J.
Piotrowska-Seget, Zofia
Keywords: BIOLOG-CLPP; DGGE; Heavy metals; HPLC-MS; Metallophytes; PLFA; Rhizosphere biodiversity
Issue Date: 2018
Citation: Frontiers in Microbiology, Vol. 9, Iss. JUL (2018), Art. No. 1425
Abstract: We here assess the biodiversity of the rhizosphere microbial communities of metal-tolerant plant species Arabidopsis arenosa, Arabidopsis halleri, Deschampsia caespitosa, and Silene vulgaris when growing on various heavy metal polluted sites. Our broad-spectrum analyses included counts for total and metal-tolerant culturable bacteria, assessments of microbial community structure by phospholipid fatty acid (PLFA) profiling and community-level analysis based on BIOLOG-CLPP to indicate functional diversity. The genetic-biochemical diversity was also measured by denaturing gradient gel electrophoresis (PCR-DGGE) and metabolomic analysis (HPLC-MS). Different rhizospheres showed distinctive profiles of microbial traits, which also differed significantly from bulk soil, indicating an influence from sampling site as well as plant species. However, total bacterial counts and PCR-DGGE profiles were most affected by the plants, whereas sampling site-connected variability was predominant for the PLFA profiles and an interaction of both factors for BIOLOG-CLPP. Correlations were also observed between pH, total and bioavailable Cd or Zn and measured microbial traits. Thus, both plant species and heavy-metals were shown to be major determinants of microbial community structure and function.
DOI: 10.3389/fmicb.2018.01425
ISSN: 1664-302X
Appears in Collections:Artykuły (WNP)

Files in This Item:
File Description SizeFormat 
Borymski_Plant_species_and_heavy_metals_affect_biodiversity.pdf1,88 MBAdobe PDFView/Open
Show full item record

Uznanie Autorstwa 3.0 Polska Creative Commons License Creative Commons