DC pole | Wartość | Język |
dc.contributor.author | Wójcik, Anna M. | - |
dc.contributor.author | Nodine, Michael D. | - |
dc.contributor.author | Gaj, Małgorzata D. | - |
dc.date.accessioned | 2019-04-11T12:03:57Z | - |
dc.date.available | 2019-04-11T12:03:57Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Frontiers in Plant Science, Vol. 8 (2017), art. no. 2024 | pl_PL |
dc.identifier.issn | 1664-462X | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/8806 | - |
dc.description.abstract | MicroRNAs are non-coding small RNA molecules that are involved in the
post-transcriptional regulation of the genes that control various developmental
processes in plants, including zygotic embryogenesis (ZE). miRNAs are also believed
to regulate somatic embryogenesis (SE), a counterpart of the ZE that is induced in
vitro in plant somatic cells. However, the roles of specific miRNAs in the regulation of
the genes involved in SE, in particular those encoding transcription factors (TFs) with
an essential function during SE including LEAFY COTYLEDON2 (LEC2), remain mostly
unknown. The aim of the study was to reveal the function of miR165/166 and miR160
in the LEC2-controlled pathway of SE that is induced in in vitro cultured Arabidopsis
explants.In ZE, miR165/166 controls the PHABULOSA/PHAVOLUTA (PHB/PHV) genes,
which are the positive regulators of LEC2, while miR160 targets the AUXIN RESPONSE
FACTORS (ARF10, ARF16, ARF17) that control the auxin signaling pathway, which plays
key role in LEC2-mediated SE. We found that a deregulated expression/function of
miR165/166 and miR160 resulted in a significant accumulation of auxin in the cultured
explants and the spontaneous formation of somatic embryos. Our results show that
miR165/166 might contribute to SE induction via targeting PHB, a positive regulator of
LEC2 that controls embryogenic induction via activation of auxin biosynthesis pathway
(Wójcikowska et al., 2013). Similar to miR165/166, miR160 was indicated to control
SE induction through auxin-related pathways and the negative impact of miR160 on
ARF10/ARF16/ARF17 was shown in an embryogenic culture. Altogether, the results
suggest that the miR165/166- and miR160-node contribute to the LEC2-mediated
auxin-related pathway of embryogenic transition that is induced in the somatic cells of
Arabidopsis. A model summarizing the suggested regulatory interactions between the
miR165/166-PHB and miR160-ARF10/ARF16/ARF17 nodes that control SE induction
in Arabidopsis was proposed. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | AUXIN RESPONSE FACTOR10 | pl_PL |
dc.subject | ARF16 | pl_PL |
dc.subject | ARF17 | pl_PL |
dc.subject | LEAFY COTYLEDON2 | pl_PL |
dc.subject | miRNA | pl_PL |
dc.subject | PHABULOSA | pl_PL |
dc.subject | PHAVOLUTA | pl_PL |
dc.subject | somatic embryogenesis | pl_PL |
dc.title | miR160 and miR166/165 Contribute to the LEC2-Mediated Auxin Response Involved in the Somatic Embryogenesis Induction in Arabidopsis | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.relation.journal | Frontiers in Plant Science | pl_PL |
Pojawia się w kolekcji: | Artykuły (WNP)
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