DC pole | Wartość | Język |
dc.contributor.author | Guzik, Urszula | - |
dc.contributor.author | Hupert-Kocurek, Katarzyna | - |
dc.contributor.author | Sitnik, Małgorzata | - |
dc.contributor.author | Wojcieszyńska, Danuta | - |
dc.date.accessioned | 2019-05-10T08:24:59Z | - |
dc.date.available | 2019-05-10T08:24:59Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Antonie van Leeuwenhoek, Vol. 103, iss. 6 (2013), s. 1297-1307 | pl_PL |
dc.identifier.issn | 0003-6072 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/9080 | - |
dc.description.abstract | This is the first report of a catechol 1,2-
dioxygenase from Stenotrophomonas maltophilia
strain KB2 with high activity against catechol and its
methyl derivatives. This enzyme was maximally
active at pH 8.0 and 40 C and the half-life of the
enzyme at this temperature was 3 h. Kinetic studies
showed that the value of Km and Vmax was 12.8 lM
and 1,218.8 U/mg of protein, respectively. During our
studies on kinetic properties of the catechol 1,2-
dioxygenase we observed substrate inhibition at
[80 lM. The nucleotide sequence of the gene
encoding the S. maltophilia strain KB2 catechol 1,2-
dioxygenase has high identity with other catA genes
from members of the genus Pseudomonas. The
deduced 314-residue sequence of the enzyme corresponds
to a protein of molecular mass 34.5 kDa. This
enzyme was inhibited by competitive inhibitors (phenol
derivatives) only by ca. 30 %. High tolerance
against condition changes is desirable in industrial
processes. Our data suggest that this enzyme could be
of use as a tool in production of cis,cis-muconic acid
and its derivatives. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | cis,cis-muconic acid production | pl_PL |
dc.subject | Stenotrophomonas | pl_PL |
dc.subject | Catechol 1,2-dioxygenase | pl_PL |
dc.subject | Substrate specificity | pl_PL |
dc.subject | Kinetic | pl_PL |
dc.title | High activity catechol 1,2-dioxygenase from Stenotrophomonas maltophilia strain KB2 as a useful tool in cis,cis-muconic acid production | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.relation.journal | Antonie van Leeuwenhoek | pl_PL |
dc.identifier.doi | 10.1007/s10482-013-9910-8 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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