DC pole | Wartość | Język |
dc.contributor.author | Kwaśniewska, Jolanta | - |
dc.contributor.author | Kwaśniewski, Mirosław | - |
dc.date.accessioned | 2019-06-04T09:50:12Z | - |
dc.date.available | 2019-06-04T09:50:12Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Journal of Applied Genetics, 2013, no. 4, s. 407-415 | pl_PL |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/9346 | - |
dc.description.abstract | The aim of this study was to perform a comparative
investigation of the actions of three mutagens that are widely
used in plantmutagenesis using the comet-FISH technique. The
comet-FISH technique was used for the analysis of DNA
damage and the kinetics of repair within specific DNA sequences.
FISH with rDNA and telomeric/centromeric DNA
probes was applied to comets that were obtained from an
alkaline/neutral comet assay. Migration within specific DNA
sequences was analysed after treatment with two chemical
mutagens-maleic hydrazide (MH) and N-nitroso-N-methylurea
(MNU), and γ-rays. Barley was used as a model plant in this
study. The possible utility of specific DNA sequences in a
comparative assessment of the distribution of DNA damage
within a plant genome was evaluated. This study proved that
the comet-FISH technique is suitable for a detailed quantification
ofDNA damage and repairwithin specificDNA sequences
in plant mutagenesis. The analysis of FISH signals demonstrated
that the involvement of specific DNA sequences in DNA
damage was different and was dependent on the mutagen used.
We showed that 5S rDNA and telomeric DNA sequences are
more sensitive to mutagenic treatment, which was expressed by
a stronger fragmentation and migration in comparison to the
other probes used in the study. We found that 5S rDNA and
telomeric DNA probes are more suitable for testing the
genotoxicity of environmental factors. A comparison of the
involvement of specific chromosome domains in direct DNA
breakage/repair and in chromosome aberration formation after
mutagen treatment indicates the compatibility of the results. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | Barley | pl_PL |
dc.subject | Centromere | pl_PL |
dc.subject | DNA fragmentation | pl_PL |
dc.subject | rDNA | pl_PL |
dc.subject | Telomere | pl_PL |
dc.title | Comet-FISH for the evaluation of plant DNA damage after mutagenic treatments | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.relation.journal | Journal of Applied Genetics | pl_PL |
dc.identifier.doi | 10.1007/s13353-013-0169-6 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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