DC pole | Wartość | Język |
dc.contributor.author | Gliwicka, Marta | - |
dc.contributor.author | Nowak, Katarzyna | - |
dc.contributor.author | Cieśla, Edyta | - |
dc.contributor.author | Gaj, Małgorzata D. | - |
dc.date.accessioned | 2019-06-04T09:53:24Z | - |
dc.date.available | 2019-06-04T09:53:24Z | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | Plant Cell, Tissue and Organ Culture, Vol. 109 (2012), s. 235–245 | pl_PL |
dc.identifier.issn | 0167-6857 | - |
dc.identifier.issn | 1573-5044 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/9347 | - |
dc.description.abstract | In Arabidopsis, immature zygotic embryos
(IZEs) at the late cotyledon stage provide the only explants
that can be used to induce in vitro somatic embryogenesis
(SE) with high efficiency. The most conspicuous characteristic
of SE-competent IZEs is the accumulation of seed
storage reserves (SSR), as proteins and lipids. In order to
elucidate an assumed role of these compounds in the
mechanisms involved in tissue capacity for SE, the genes
encoding the main seed storage protein, cruciferin (CRUCIFERIN1,
CRA1) and the lipid body-related protein
oleosin (OLEOSIN4, OLEO4), were studied. Significantly
higher transcriptional activity of both genes, CRA1 and
OLEO4, in embryogenic than in non-embryogenic cultures,
were indicated. However, their activity under in vitro culture
were found not to be induced by auxin treatment or
LEC2 expression, and were unspecific for SE induction. In
addition, the results on mutants severely impaired in SE
response indicated that high activity of SSR genes in
explant tissue is not sufficient for SE induction. On the
other hand, the cra1 and oleo4 insertional mutants were
found to be defective in their capacity for SE. In addition, it
was found that the mutants displayed lower tolerance to
high salinity and osmotic stress. Altogether, the results
suggest an indirect influence of SSR genes on the
embryogenic capacity of cultured tissues possibly via
improvement of cell response to stress imposed in vitro. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa-Użycie niekomercyjne 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/3.0/pl/ | * |
dc.subject | CRUCIFERIN1 | pl_PL |
dc.subject | Immature zygotic embryos | pl_PL |
dc.subject | LEC2 overexpression | pl_PL |
dc.subject | OLEOSIN4 | pl_PL |
dc.subject | Real-time qPCR | pl_PL |
dc.subject | Shoot organogenesis | pl_PL |
dc.subject | Somatic embryogenesis | pl_PL |
dc.subject | SE-impaired mutants | pl_PL |
dc.title | Expression of seed storage product genes (CRA1 and OLEO4) in embryogenic cultures of somatic tissues of Arabidopsis | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.1007/s11240-011-0089-2 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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