DC pole | Wartość | Język |
dc.contributor.author | Gruszka, Dariusz | - |
dc.contributor.author | Szarejko, Iwona | - |
dc.contributor.author | Małuszyński, Mariusz | - |
dc.date.accessioned | 2019-06-04T12:25:06Z | - |
dc.date.available | 2019-06-04T12:25:06Z | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | Plant Growth Regulation, Vol. 65 (2011), s. 343–358 | pl_PL |
dc.identifier.issn | 0167-6903 | - |
dc.identifier.issn | 1573-5087 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/9356 | - |
dc.description.abstract | Brassinosteroids (BR) are plant steroid hormones
regulating various aspects of morphogenesis, such
as seed development and germination, cell division and
elongation, differentiation of tracheary elements, development
during growth in darkness (skotomorphogenesis),
photosynthesis and response to environmental stress.
Brassinosteroid synthesis has been studied to a great extent
in the dicot species, Arabidopsis thaliana, resulting in the
identification of genes participating in this process. Much
less is known about BR synthesis in crops, including the
monocots. The purpose of this study was to identify and
characterize barley coding sequence HvDWARF involved
in brassinosteroid synthesis. This sequence, encoding
brassinosteroid-6-oxidase, was identified on the basis of
barley ESTs. This sequence was screened for nucleotide
substitutions in semi-dwarf, chemically-induced barley
mutants exhibiting changes in etiolation. The responsiveness
of these genotypes to exogenous brassinosteroids was
determined with the use of leaf-blade segment unrolling
tests. The semi-dwarf phenotype of the BR-deficient
mutants was rescued by the application of 10-5 M 24-epibrassinolide.
Two missense mutations were identified
within the HvDWARF sequence in BR-deficient mutants
522DK and 527DK from variety ‘Delisa’. These substitutions
cause changes of amino acid residues located within
the conserved fragments of the encoded polypeptide. Thetranscription profile of HvDWARF and HvBAK1/SERK3,
involved in BR signaling, was determined during the early
stages of seedling development in BR-deficient and BRinsensitive
mutants using real-time quantitative PCR. This
analysis indicated that HvDWARF displays a uniformly
low level of this process, whereas the transcription level of
HvBAK1 proved to be spatially and temporally regulated. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa-Użycie niekomercyjne 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/3.0/pl/ | * |
dc.subject | Barley | pl_PL |
dc.subject | Brassinosteroids | pl_PL |
dc.subject | Synthesis | pl_PL |
dc.subject | Semi-dwarf | pl_PL |
dc.subject | Mutants | pl_PL |
dc.title | Identification of barley DWARF gene involved in brassinosteroid synthesis | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.1007/s10725-011-9607-9 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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