DC pole | Wartość | Język |
dc.contributor.author | Kurowska, Marzena | - |
dc.contributor.author | Daszkowska-Golec, Agata | - |
dc.contributor.author | Gruszka, Damian | - |
dc.contributor.author | Marzec, Marek | - |
dc.contributor.author | Szurman, M. | - |
dc.contributor.author | Szarejko, Iwona | - |
dc.contributor.author | Małuszyński, Mirosław | - |
dc.date.accessioned | 2019-06-11T09:46:26Z | - |
dc.date.available | 2019-06-11T09:46:26Z | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | Journal of Applied Genetics, Vol. 52 (2011), s. 371-390 | pl_PL |
dc.identifier.issn | 1234-1983 | - |
dc.identifier.issn | 2190-3883 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/9437 | - |
dc.description.abstract | Recent advances in large-scale genome sequencing
projects have opened up new possibilities for the application
of conventional mutation techniques in not only forward but
also reverse genetics strategies. TILLING (Targeting Induced
Local Lesions IN Genomes) was developed a decade ago as
an alternative to insertional mutagenesis. It takes advantage of
classical mutagenesis, sequence availability and highthroughput
screening for nucleotide polymorphisms in a
targeted sequence. The main advantage of TILLING as a
reverse genetics strategy is that it can be applied to any
species, regardless of its genome size and ploidy level. The
TILLING protocol provides a high frequency of point
mutations distributed randomly in the genome. The great
mutagenic potential of chemical agents to generate a high rate
of nucleotide substitutions has been proven by the high
density of mutations reported for TILLING populations in
various plant species. Formost of them, the analysis of several
genes revealed 1 mutation/200–500 kb screened and much
higher densities were observed for polyploid species, such as
wheat. High-throughput TILLING permits the rapid and lowcost
discovery of new alleles that are induced in plants.
Several research centres have established a TILLING public
service for various plant species. The recent trends in
TILLING procedures rely on the diversification of bioinformatic
tools, new methods of mutation detection, including
mismatch-specific and sensitive endonucleases, but also
various alternatives for LI-COR screening and single nucleotide
polymorphism (SNP) discovery using next-generation sequencing technologies. The TILLING strategy has found
numerous applications in functional genomics. Additionally,
wide applications of this throughput method in basic and
applied research have already been implemented through
modifications of the original TILLING strategy, such as
Ecotilling or Deletion TILLING. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa-Użycie niekomercyjne 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/3.0/pl/ | * |
dc.subject | TILLING platform | pl_PL |
dc.subject | Plant mutagenesis | pl_PL |
dc.subject | Bioinformatic tools | pl_PL |
dc.subject | Reverse genetics | pl_PL |
dc.subject | Next-generation sequencing technologies | pl_PL |
dc.title | TILLING - a shortcut in functional genomics | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.1007/s13353-011-0061-1 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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