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Title: Creation of a TILLING population in barley after chemical mutagenesis with sodium azide and MNU
Authors: Szarejko, Iwona
Szurman-Zubrzycka, Miriam
Nawrot, Małgorzata
Marzec, Marek
Gruszka, Damian
Kurowska, Marzena
Chmielewska, Beata
Zbieszczyk, Justyna
Jelonek, Janusz
Małuszyński, Mirosław
Keywords: Chemical mutagenesis; Alkylating agents; MNU; Sodium azide; TILLING; Barley; Phenotyping; Database
Issue Date: 2017
Citation: J. Jankowicz-Cieslak, T. Tai, J. Kumlehn, B. Till (red.), “Biotechnologies for Plant Mutation Breeding” (S. 91-111). Cham : Springer
Abstract: Since the development of the Targeting Induced Local Lesions in Genome (TILLING) strategy, it has been applied in both plants and animals in many studies. The creation of an appropriate population is the first and most crucial step of TILLING. The goal is to obtain a highly mutagenized population that allows many mutations in any gene of interest to be found. Therefore, an effective method of mutation induction should be developed. A high mutation density is associated with saving time, costs, and the labor required for the development of a TILLING platform. The proper handling of the mutated generations, the establishment of a seed bank, and the development of a DNA library are essential for creating a TILLING population. The database in which all of the data from the molecular and phenotypic analyses are collected is a very useful tool for maintaining such population. Once developed, a TILLING population can serve as a renewable resource of mutations for research that uses both forward and reverse genetic approaches. In this chapter, we describe the methods for the development and maintenance of a TILLING population in barley.
DOI: 10.1007/978-3-319-45021-6_6
ISBN: 978-3-319-45019-3
ISSN: 978-3-319-45021-6
Appears in Collections:Książki/rozdziały (WNP)

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