DC pole | Wartość | Język |
dc.contributor.author | Desta, Zeratsion Abera | - |
dc.contributor.author | Kolano, Bożena | - |
dc.contributor.author | Shamim, Zeeshan | - |
dc.contributor.author | Armstrong, Susan J. | - |
dc.contributor.author | Rewers, Monika | - |
dc.contributor.author | Śliwińska, Estera | - |
dc.contributor.author | Kushwaha, Kumar | - |
dc.contributor.author | Parkin, Isobel A.P. | - |
dc.contributor.author | Ortiz, Rodomiro | - |
dc.contributor.author | de Koning, Dirk-Jan | - |
dc.date.accessioned | 2019-11-22T20:05:16Z | - |
dc.date.available | 2019-11-22T20:05:16Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Scientific Reports (Nature Publishing Group), (2019), iss. 9., art no. 17028 | pl_PL |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/11691 | - |
dc.description.abstract | Field cress (Lepidium campestre L.), despite its potential as a sustainable alternative oilseed plant,
has been underutilized, and no prior attempts to characterize the genome at the genetic or molecular
cytogenetic level have been conducted. Genetic maps are the foundation for anchoring and orienting
annotated genome assemblies and positional cloning of candidate genes. Our principal goal was to
construct a genetic map using integrated approaches of genetic, comparative and cytogenetic map
analyses. In total, 503 F2 interspecific hybrid individuals were genotyped using 7,624 single nucleotide
polymorphism markers. Comparative analysis demonstrated that ~57% of the sequenced loci in L.
campestre were congruent with Arabidopsis thaliana (L.) genome and suggested a novel karyotype,
which predates the ancestral crucifer karyotype. Aceto-orcein chromosome staining and fluorescence
in situ hybridization (FISH) analyses confirmed that L. campestre, L. heterophyllum Benth. and their
hybrids had a chromosome number of 2n = 2x = 16. Flow cytometric analysis revealed that both species
possess 2C roughly 0.4 picogram DNA. Integrating linkage and comparative maps with cytogenetic map
analyses assigned two linkage groups to their particular chromosomes. Future work could incorporate
FISH utilizing A. thaliana mapped BAC clones to allow the chromosomes of field cress to be identified
reliably. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | Cytogenetic analysis | pl_PL |
dc.subject | Lepidium campestre | pl_PL |
dc.subject | rDNA | pl_PL |
dc.subject | Genome analysis | pl_PL |
dc.subject | Field cress | pl_PL |
dc.subject | Genetic map | pl_PL |
dc.title | Field cress genome mapping: Integrating linkage and comparative maps with cytogenetic analysis for rDNA carrying chromosomes | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.1038/s41598-019-53320-0 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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