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Title: In Vitro Tissue Culture in Brachypodium: Applications and Challenges
Authors: Betekhtin, Alexander
Hus, Karolina
Rojek-Jelonek, Magdalena
Kurczyńska, Ewa
Nibau, Candida
Doonan, John H.
Hasterok, Robert
Keywords: Agrobacterium; Brachypodium distachyon; Brachypodium species; cell wall; genes; model plant; somatic embryogenesis; transformation
Issue Date: 2020
Citation: International Journal of Molecular Sciences, 2020, vol. 21, art. no. 1037, p. 1-16
Abstract: Brachypodium distachyon has become an excellent model for plant breeding and bioenergy grasses that permits many fundamental questions in grass biology to be addressed. One of the constraints to performing research in many grasses has been the di culty with which they can be genetically transformed and the generally low frequency of such transformations. In this review, we discuss the contribution that transformation techniques have made in Brachypodium biology as well as how Brachypodium could be used to determine the factors that might contribute to transformation efficiency. In particular, we highlight the latest research on the mechanisms that govern the gradual loss of embryogenic potential in a tissue culture and propose using B. distachyon as a model for other recalcitrant monocots.
DOI: 10.3390/ijms21031037
ISSN: 1422-0067
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