http://hdl.handle.net/20.500.12128/13954
Tytuł: | The Impact of Mutations in the HvCPD and HvBRI1 Genes on the Physicochemical Properties of the Membranes from Barley Acclimated to Low/High Temperatures |
Autor: | Rudolphi-Szydło, Elżbieta Sadura, Iwona Filek, Maria Gruszka, Damian Janeczko, Anna |
Słowa kluczowe: | galactolipids; phospholipids; plant acclimation to high/low temperature; brassinosteroids; cell membranes; Langmuir monolayers |
Data wydania: | 2020 |
Źródło: | "Cells" Vol. 9, iss. 5 (2020), art. no 1125 |
Abstrakt: | 1) Background: The study characterized barley mutants with brassinosteroid (BR) biosynthesis and signaling disturbances in terms of the physicochemical/structural properties of membranes to enrich the knowledge about the role of brassinosteroids for lipid metabolism and membrane functioning. (2) Methods: The Langmuir method was used to investigate the properties of the physicochemical membranes. Langmuir monolayers were formed from the lipid fractions isolated from the plants growing at 20 C and then acclimated at 5 C or 27 C. The fatty acid composition of the lipids was estimated using gas chromatography. (3) Results: The BR-biosynthesis and BR-signaling mutants of barley were characterized by a temperature-dependent altered molar percentage of fatty acids (from 14:0 to 20:1) in their galactolipid and phospholipid fractions in comparison to wild-type (WT). For example, the mutants had a lower molar percentage of 18:3 in the phospholipid (PL) fraction. The same regularity was observed at 5 C. It resulted in altered physicochemical parameters of the membranes (Alim, coll, Cs1). (4) Conclusions: BR may be involved in regulating fatty acid biosynthesis or their transport/incorporation into the cell membranes. Mutants had altered physicochemical parameters of their membranes, compared to the WT, which suggests that BR may have a multidirectional impact on the membrane-dependent physiological processes. |
URI: | http://hdl.handle.net/20.500.12128/13954 |
DOI: | 10.3390/cells9051125 |
ISSN: | 2073-4409 |
Pojawia się w kolekcji: | Artykuły (WNP) |
Plik | Opis | Rozmiar | Format | |
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Gruszka_The_impact_of_mutations_in_the_HvCPD_and_HvBRI1_genes_on_the_physicochemical.pdf | 1,13 MB | Adobe PDF | Przejrzyj / Otwórz |
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