DC pole | Wartość | Język |
dc.contributor.author | Burdach, Zbigniew | - |
dc.contributor.author | Siemieniuk, Agnieszka | - |
dc.contributor.author | Karcz, Waldemar | - |
dc.date.accessioned | 2020-07-14T06:05:11Z | - |
dc.date.available | 2020-07-14T06:05:11Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | International Journal of Molecular Sciences, Vol. 21 (2020), Art. No. 4876 | pl_PL |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/15142 | - |
dc.description.abstract | In contrast to the well-studied e ect of auxin on the plasma membrane K+ channel activity,
little is known about the role of this hormone in regulating the vacuolar K+ channels. Here, the
patch-clamp technique was used to investigate the e ect of auxin (IAA) on the fast-activating vacuolar
(FV) channels. It was found that the macroscopic currents displayed instantaneous currents, which at
the positive potentials were about three-fold greater compared to the one at the negative potentials.
When auxin was added to the bath solution at a final concentration of 1 M, it increased the outward
currents by about 60%, but did not change the inward currents. The imposition of a ten-fold
vacuole-to-cytosol KCl gradient stimulated the e ux of K+ from the vacuole into the cytosol and
reduced the K+ current in the opposite direction. The addition of IAA to the bath solution with the
10/100 KCl gradient decreased the outward current and increased the inward current. Luminal auxin
reduced both the outward and inward current by approximately 25% compared to the control.
The single channel recordings demonstrated that cytosolic auxin changed the open probability of
the FV channels at the positive voltages to a moderate extent, while it significantly increased the
amplitudes of the single channel outward currents and the number of open channels. At the positive
voltages, auxin did not change the unitary conductance of the single channels. We suggest that auxin
regulates the activity of the fast-activating vacuolar (FV) channels, thereby causing changes of the K+
fluxes across the vacuolar membrane. This mechanism might serve to tightly adjust the volume of
the vacuole during plant cell expansion. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | Beta vulgaris L. | pl_PL |
dc.subject | FV channels | pl_PL |
dc.subject | IAA | pl_PL |
dc.subject | patch-clamp | pl_PL |
dc.subject | vacuole | pl_PL |
dc.title | Effect of Auxin (IAA) on the Fast Vacuolar (FV) Channels in Red Beet (Beta vulgaris L.) Taproot Vacuoles | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.3390/ijms21144876 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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