Skip navigation

Please use this identifier to cite or link to this item:
Title: Serological characterization of the enterobacterial common antigen substitution of the lipopolysaccharide of "Yersinia enterocolitica" O:3
Authors: Noszczyńska, Magdalena
Kasperkiewicz, Katarzyna
Duda, Katarzyna A.
Podhorodecka, Joanna
Rabsztyn, Kamil
Gwizdała, Karolina
Świerzko, Anna S.
Radziejewska-Lebrecht, Joanna
Holst, Otto
Skurnik, Mikael
Keywords: Yersinia enterocolitica O : 3; whole-cell lysate; caprylic acid; electrospray ionization; enterobacterial common antigen
Issue Date: 2015
Citation: "Microbiology" pt 161, (2015), s. 219-227
Abstract: Enterobacterial common antigen (ECA) is a polysaccharide present in all members of Enterobacteriaceae anchored either via phosphatidylglycerol (PG) or LPS to the outer leaflet of the outer membrane (ECAPG and ECALPS, respectively). Only the latter form is ECAimmunogenic. We previously demonstrated that Yersinia enterocolitica O: 3 and its rough (Ospecific polysaccharide-negative) mutants were ECA-immunogenic, suggesting that they contained ECALPS; however, it was not known which part of the LPS core region was involved in ECA binding. To address this, we used a set of three deep-rough LPS mutants for rabbit immunization. The polyvalent antisera obtained were: (i) analysed for the presence of anti-LPS and anti-ECA antibodies; (ii) treated with caprylic acid (CA) to precipitate IgM antibodies and protein aggregates; and (iii) adsorbed with live ECA-negative bacteria to obtain specific anti-ECA antisera. We demonstrated the presence of antibodies specific for both ECAPG and ECALPS in all antisera obtained. Both CA treatment and adsorption with ECA-negative bacteria efficiently removed anti-LPS antibodies, resulting in specific anti-ECA sera. The LPS of the ECALPS-positive deepest-rough mutant contained only lipid A and 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) residues of the inner core, suggesting that ECALPS was linked to the Kdo region of LPS in Y. enterocolitica O:3.
DOI: 10.1099/mic.0.083493-0
ISSN: 1465-2080
Appears in Collections:Artykuły (WNP)

Files in This Item:
File Description SizeFormat 
Noszczynska_Kasperkiewicz_serological_characterization_of_the_enterobacterial.pdf687,38 kBAdobe PDFView/Open
Show full item record

Uznanie Autorstwa 3.0 Polska Creative Commons License Creative Commons