Skip navigation

Please use this identifier to cite or link to this item:
Title: Genetic and Methylome Variation in Turkish „Brachypodium Distachyon” Accessions Differentiate Two Geographically Distinct Subpopulations
Authors: Skalska, Aleksandra
Stritt, Christoph
Wyler, Michele
Williams, Hefin W.
Vickers, Martin
Han, Jiwan
Tuna, Metin
Tuna, Gulsemin Savas
Susek, Karolina
Swain, Martin
Wóycicki, Rafał K.
Chaudhary, Saurabh
Corke, Fiona
Doonan, John H.
Roulin, Anne C.
Hasterok, Robert
Mur, Louis A. J.
Keywords: Brachypodium; DNA methylation; drought; flowering; genome; phenomics
Issue Date: 2020
Citation: "International Journal of Molecular Sciences", (2020), Vol. 18, art. No. 6700, s. 1-17
Abstract: Brachypodium distachyon (Brachypodium) is a non-domesticated model grass species that can be used to test if variation in genetic sequence or methylation are linked to environmental di erences. To assess this, we collected seeds from 12 sites within five climatically distinct regions of Turkey. Seeds from each region were grown under standardized growth conditions in the UK to preserve methylated sequence variation. At six weeks following germination, leaves were sampled and assessed for genomic and DNA methylation variation. In a follow-up experiment, phenomic approaches were used to describe plant growth and drought responses. Genome sequencing and population structure analysis suggested three ancestral clusters across the Mediterranean, two of which were geographically separated in Turkey into coastal and central subpopulations. Phenotypic analyses showed that the coastal subpopulation tended to exhibit relatively delayed flowering and the central, increased drought tolerance as indicated by reduced yellowing. Genome-wide methylation analyses in GpC, CHG and CHH contexts also showed variation which aligned with the separation into coastal and central subpopulations. The climate niche modelling of both subpopulations showed a significant influence from the “Precipitation in the Driest Quarter” on the central subpopulation and “Temperature of the Coldest Month” on the coastal subpopulation. Our work demonstrates genetic diversity and variation in DNA methylation in Turkish accessions of Brachypodium that may be associated with climate variables and the molecular basis of which will feature in ongoing analyses.
DOI: 10.3390/ijms21186700
ISSN: 1422-0067
Appears in Collections:Artykuły (WNP)

Files in This Item:
File Description SizeFormat 
Skalska_Hasterok_genetic_and_methylome_variation.pdf6,34 MBAdobe PDFView/Open
Show full item record

Uznanie Autorstwa 3.0 Polska Creative Commons License Creative Commons