DC pole | Wartość | Język |
dc.contributor.author | Nowak, Katarzyna | - |
dc.contributor.author | Morończyk, Joanna | - |
dc.contributor.author | Wójcik, Anna M. | - |
dc.contributor.author | Gaj, Małgorzata D. | - |
dc.date.accessioned | 2020-09-17T11:51:06Z | - |
dc.date.available | 2020-09-17T11:51:06Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | "International Journal of Molecular Sciences" Vol. 21, iss. 18 (2020), art. no 6733 | pl_PL |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/15975 | - |
dc.description.abstract | The embryogenic transition of somatic cells requires an extensive reprogramming of
the cell transcriptome. Relevantly, the extensive modulation of the genes that have a regulatory
function, in particular the genes encoding the transcription factors (TFs) and miRNAs, have been
indicated as controlling somatic embryogenesis (SE) that is induced in vitro in the somatic cells of
plants. Identifying the regulatory relationships between the TFs and miRNAs during SE induction
is of central importance for understanding the complex regulatory interplay that fine-tunes a
cell transcriptome during the embryogenic transition. Hence, here, we analysed the regulatory
relationships between AGL15 (AGAMOUS-LIKE 15) TF and miR156 in an embryogenic culture of
Arabidopsis. Both AGL15 and miR156 control SE induction and AGL15 has been reported to target
the MIR156 genes in planta. The results showed that AGL15 contributes to the regulation of miR156
in an embryogenic culture at two levels that involve the activation of the MIR156 transcription and
the containment of the abundance of mature miR156 by repressing the miRNA biogenesis genes
DCL1 (DICER-LIKE1), SERRATE and HEN1 (HUA-ENHANCER1). To repress the miRNA biogenesis
genes AGL15 seems to co-operate with the TOPLESS co-repressors (TPL and TPR1-4), which are
components of the SIN3/HDAC silencing complex. The impact of TSA (trichostatin A), an inhibitor
of the HDAC histone deacetylases, on the expression of the miRNA biogenesis genes together with
the ChIP results implies that histone deacetylation is involved in the AGL15-mediated repression
of miRNA processing. The results indicate that HDAC6 and HDAC19 histone deacetylases might
co-operate with AGL15 in silencing the complex that controls the abundance of miR156 during
embryogenic induction. This study provides new evidence about the histone acetylation-mediated
control of the miRNA pathways during the embryogenic reprogramming of plant somatic cells and
the essential role of AGL15 in this regulatory mechanism. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | somatic embryogenesis | pl_PL |
dc.subject | AGL15 | pl_PL |
dc.subject | miR156 | pl_PL |
dc.subject | miRNA biogenesis | pl_PL |
dc.subject | acetylation | pl_PL |
dc.subject | HDAC | pl_PL |
dc.subject | TOPLESS co-repressor | pl_PL |
dc.subject | HEN1 | pl_PL |
dc.subject | SERRATE | pl_PL |
dc.subject | DCL1 | pl_PL |
dc.title | AGL15 Controls the Embryogenic Reprogramming of Somatic Cells in Arabidopsis through the Histone Acetylation-Mediated Repression of the miRNA Biogenesis Genes | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.3390/ijms21186733 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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