DC pole | Wartość | Język |
dc.contributor.author | Grzybkowska, Daria | - |
dc.contributor.author | Nowak, Katarzyna | - |
dc.contributor.author | Gaj, Małgorzata D. | - |
dc.date.accessioned | 2020-09-22T07:18:49Z | - |
dc.date.available | 2020-09-22T07:18:49Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | International Journal of Molecular Sciences, Vol. 21 (2020), Art. No. 6849 | pl_PL |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/16054 | - |
dc.description.abstract | The auxin-induced embryogenic reprogramming of plant somatic cells is associated with
extensive modulation of the gene expression in which epigenetic modifications, including DNA
methylation, seem to play a crucial role. However, the function of DNA methylation, including
the role of auxin in epigenetic regulation of the SE-controlling genes, remains poorly understood.
Hence, in the present study, we analysed the expression and methylation of the TF genes that play a
critical regulatory role during SE induction (LEC1, LEC2, BBM, WUS and AGL15) in auxin-treated
explants of Arabidopsis. The results showed that auxin treatment substantially a ected both the
expression and methylation patterns of the SE-involved TF genes in a concentration-dependent
manner. The auxin treatment di erentially modulated the methylation of the promoter (P) and gene
body (GB) sequences of the SE-involved genes. Relevantly, the SE-e ective auxin treatment (5.0 M
of 2,4-D) was associated with the stable hypermethylation of the P regions of the SE-involved genes
and a significantly higher methylation of the P than the GB fragments was a characteristic feature of
the embryogenic culture. The presence of auxin-responsive (AuxRE) motifs in the hypermethylated P
regions suggests that auxin might substantially contribute to the DNA methylation-mediated control
of the SE-involved genes. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | DNA methylation | pl_PL |
dc.subject | somatic embryogenesis | pl_PL |
dc.subject | SE-related genes | pl_PL |
dc.title | Hypermethylation of Auxin-Responsive Motifs in the Promoters of the Transcription Factor Genes Accompanies the Somatic Embryogenesis Induction in Arabidopsis | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.3390/ijms21186849 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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