http://hdl.handle.net/20.500.12128/17055
Tytuł: | Al-Tolerant Barley Mutant hvatr.g Shows the ATR-Regulated DNA Damage Response to Maleic Acid Hydrazide |
Autor: | Jaśkowiak, Joanna Kwaśniewska, Jolanta Szurman-Zubrzycka, Miriam Rojek-Jelonek, Magdalena Larsen, Paul B. Szarejko, Iwona |
Słowa kluczowe: | aluminum toxicity; ATR; barley; cell cycle; DDR pathway; DNA damage; maleic acid hydrazide |
Data wydania: | 2020 |
Źródło: | "International Journal of Molecular Sciences" Vol. 21, iss. 22 (2020), art. no. 8500 |
Abstrakt: | ATR, a DNA damage signaling kinase, is required for cell cycle checkpoint regulation and detecting DNA damage caused by genotoxic factors including Al3+ ions. We analyzed the function of the HvATR gene in response to chemical clastogen-maleic acid hydrazide (MH). For this purpose, the Al-tolerant barley TILLING mutant hvatr.g was used. We described the e ects of MH on the nuclear genome of hvatr.g mutant and its WT parent cv. “Sebastian”, showing that the genotoxic e ect measured by TUNEL test and frequency of cells with micronuclei was much stronger in hvatr.g than in WT. MH caused a significant decrease in the mitotic activity of root cells in both genotypes, however this e ect was significantly stronger in “Sebastian”. The impact of MH on the roots cell cycle, analyzed using flow cytometry, showed no di erences between the mutant and WT. |
URI: | http://hdl.handle.net/20.500.12128/17055 |
DOI: | 10.3390/ijms21228500 |
ISSN: | 1422-0067 |
Pojawia się w kolekcji: | Artykuły (WNP) |
Plik | Opis | Rozmiar | Format | |
---|---|---|---|---|
Jaskowiak_AI-tolerant_barley_mutant_havtrg_shows_the_ATR-regulated_DNA_damage.pdf | 1,11 MB | Adobe PDF | Przejrzyj / Otwórz |
Uznanie Autorstwa 3.0 Polska Creative Commons