DC pole | Wartość | Język |
dc.contributor.author | Wójcik-Jagła, Magdalena | - |
dc.contributor.author | Daszkowska-Golec, Agata | - |
dc.contributor.author | Fiust, Anna | - |
dc.contributor.author | Kopeć, Przemysław | - |
dc.contributor.author | Rapacz, Marcin | - |
dc.date.accessioned | 2021-02-18T14:21:12Z | - |
dc.date.available | 2021-02-18T14:21:12Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | International Journal of Molecular Scence" (2021), Vol. 22, art. no. 1057 | pl_PL |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/19140 | - |
dc.description.abstract | Mechanisms involved in the de-acclimation of herbaceous plants caused by warm periods
during winter are poorly understood. This study identifies the genes associated with this mechanism
in winter barley. Seedlings of eight accessions (four tolerant and four susceptible to de-acclimation
cultivars and advanced breeding lines) were cold acclimated for three weeks and de-acclimated
at 12 C/5 C (day/night) for one week. We performed differential expression analysis using
RNA sequencing. In addition, reverse-transcription quantitative real-time PCR and enzyme activity
analyses were used to investigate changes in the expression of selected genes. The number of
transcripts with accumulation level changed in opposite directions during acclimation and deacclimation
was much lower than the number of transcripts with level changed exclusively during
one of these processes. The de-acclimation-susceptible accessions showed changes in the expression
of a higher number of functionally diverse genes during de-acclimation. Transcripts associated
with stress response, especially oxidoreductases, were the most abundant in this group. The results
provide novel evidence for the distinct molecular regulation of cold acclimation and de-acclimation.
Upregulation of genes controlling developmental changes, typical for spring de-acclimation, was
not observed during mid-winter de-acclimation. Mid-winter de-acclimation seems to be perceived
as an opportunity to regenerate after stress. Unfortunately, it is competitive to remain in the coldacclimated
state. This study shows that the response to mid-winter de-acclimation is far more
expansive in de-acclimation-susceptible cultivars, suggesting that a reduced response to the rising
temperature is crucial for de-acclimation tolerance. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | de-acclimation | pl_PL |
dc.subject | freezing tolerance | pl_PL |
dc.subject | climate change | pl_PL |
dc.subject | barley | pl_PL |
dc.subject | RNAseq | pl_PL |
dc.subject | gene expression | pl_PL |
dc.subject | oxidoreductase | pl_PL |
dc.title | Identification of the Genetic Basis of Response to de-Acclimation in Winter Barley | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.3390/ijms22031057 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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