DC pole | Wartość | Język |
dc.contributor.author | Borowska-Zuchowska, Natalia | - |
dc.contributor.author | Robaszkiewicz, Ewa | - |
dc.contributor.author | Mykhailyk, Serhii | - |
dc.contributor.author | Wartini, Joanna | - |
dc.contributor.author | Piński, Artur | - |
dc.contributor.author | Kovarik, Ales | - |
dc.contributor.author | Hasterok, Robert | - |
dc.date.accessioned | 2021-12-06T14:49:58Z | - |
dc.date.available | 2021-12-06T14:49:58Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | "Frontier in Plant Science" (2021), Vol. 12, art. no. 768347, s. 1-13 | pl_PL |
dc.identifier.issn | 1664-462X | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/22036 | - |
dc.description.abstract | Nucleolar dominance (ND) is an epigenetic, developmentally regulated phenomenon
that describes the selective inactivation of 35S rDNA loci derived from one progenitor
of a hybrid or allopolyploid. The presence of ND was documented in an allotetraploid
grass, Brachypodium hybridum (genome composition DDSS), which is a polyphyletic
species that arose from crosses between two putative ancestors that resembled the
modern B. distachyon (DD) and B. stacei (SS). In this work, we investigated the
developmental stability of ND in B. hybridum genotype 3-7-2 and compared it with
the reference genotype ABR113. We addressed the question of whether the ND is
established in generative tissues such as pollen mother cells (PMC). We examined
condensation of rDNA chromatin by fluorescence in situ hybridization employing stateof-
art confocal microscopy. The transcription of rDNA homeologs was determined by
reverse-transcription cleaved amplified polymorphic sequence analysis. In ABR113, the
ND was stable in all tissues analyzed (primary and adventitious root, leaf, and spikes).
In contrast, the 3-7-2 individuals showed a strong upregulation of the S-genome units
in adventitious roots but not in other tissues. Microscopic analysis of the 3-7-2 PMCs
revealed extensive decondensation of the D-genome loci and their association with the
nucleolus in meiosis. As opposed, the S-genome loci were always highly condensed and
localized outside the nucleolus. These results indicate that genotype-specific loss of ND
in B. hybridum occurs probably after fertilization during developmental processes. This
finding supports our view that B. hybridum is an attractive model to study ND in grasses. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | nucleolar dominance | pl_PL |
dc.subject | 35S rDNA | pl_PL |
dc.subject | secondary constriction | pl_PL |
dc.subject | Brachypodium | pl_PL |
dc.subject | allopolyploidy | pl_PL |
dc.subject | 3D-FISH | pl_PL |
dc.subject | rRNA gene expression | pl_PL |
dc.title | To Be or Not to Be Expressed: The First Evidence of a Nucleolar Dominance Tissue-Specificity in Brachypodium hybridum | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.3389/fpls.2021.768347 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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