Skip navigation

Zastosuj identyfikator do podlinkowania lub zacytowania tej pozycji:
Tytuł: Towards the identification of new genes involved in ABA-dependent abiotic stresses using Arabidopsis suppressor mutants of abh1 hypersensitivity to ABA during seed germination
Autor: Daszkowska-Golec, Agata
Chorąży, Edyta
Małuszyński, Mirosław
Szarejko, Iwona
Słowa kluczowe: Arabidopsis; Arabidopsis Proteins; Germination; Mutation
Data wydania: 2013
Źródło: International Journal of Molecular Sciences, vol. 14, iss. 7 (2013), s. 13403-13432
Abstrakt: Abscisic acid plays a pivotal role in the abiotic stress response in plants. Although great progress has been achieved explaining the complexity of the stress and ABA signaling cascade, there are still many questions to answer. Mutants are a valuable tool in the identification of new genes or new alleles of already known genes and in elucidating their role in signaling pathways. We applied a suppressor mutation approach in order to find new components of ABA and abiotic stress signaling in Arabidopsis. Using the abh1 (ABA hypersensitive 1) insertional mutant as a parental line for EMS mutagenesis, we selected several mutants with suppressed hypersensitivity to ABA during seed germination. Here, we present the response to ABA and a wide range of abiotic stresses during the seed germination and young seedling development of two suppressor mutants-soa2 (suppressor of abh1 hypersensitivity to ABA 2) and soa3 (suppressor of abh1 hypersensitivity to ABA 3). Generally, both mutants displayed a suppression of the hypersensitivity of abh1 to ABA, NaCl and mannitol during germination. Both mutants showed a higher level of tolerance than Columbia-0 (Col-0-the parental line of abh1) in high concentrations of glucose. Additionally, soa2 exhibited better root growth than Col-0 in the presence of high ABA concentrations. soa2 and soa3 were drought tolerant and both had about 50% fewer stomata per mm2 than the wild-type but the same number as their parental line-abh1. Taking into account that suppressor mutants had the same genetic background as their parental line-abh1, it was necessary to backcross abh1 with Landsberg erecta four times for the map-based cloning approach. Mapping populations, derived from the cross of abh1 in the Landsberg erecta background with each suppressor mutant, were created. Map based cloning in order to identify the suppressor genes is in progress.
DOI: 10.3390/ijms140713403
ISSN: 1422-0067
Pojawia się w kolekcji:Artykuły (WBiOŚ)

Pliki tej pozycji:
Plik Opis RozmiarFormat 
Daszkowska_Golec_Towards_the_identification_of_new_genes_involved_in.pdf1,37 MBAdobe PDFPrzejrzyj / Otwórz
Pokaż pełny rekord

Uznanie Autorstwa 3.0 Polska Creative Commons Creative Commons