DC pole | Wartość | Język |
dc.contributor.author | Łusińska, Joanna | - |
dc.contributor.author | Majka, Joanna | - |
dc.contributor.author | Betekhtin, Alexander | - |
dc.contributor.author | Susek, Karolina | - |
dc.contributor.author | Wolny, Elżbieta | - |
dc.contributor.author | Hasterok, Robert | - |
dc.date.accessioned | 2018-09-14T11:38:25Z | - |
dc.date.available | 2018-09-14T11:38:25Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Annals of Botany, Vol. 122 (2018), s. 445–459 | pl_PL |
dc.identifier.issn | 0305-7364 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/6198 | - |
dc.description.abstract | Background and Aims The Brachypodium genus represents a useful model system to study grass genome
organization. Palaeogenomic analyses (e.g. Murat F, Armero A, Pont C, Klopp C, Salse J. 2017. Reconstructing
the genome of the most recent common ancestor of flowering plants. Nature Genetics 49: 490–496) have identified
polyploidization and dysploidy as the prime mechanisms driving the diversity of plant karyotypes and nested
chromosome fusions (NCFs) crucial for shaping grass chromosomes. This study compares the karyotype structure
and evolution in B. distachyon (genome Bd), B. stacei (genome Bs) and in their putative allotetraploid B. hybridum
(genomes BdBs).
• Methods Brachypodium chromosomes were measured and identified using multicolour fluorescence in situ
hybridization (mcFISH). For higher resolution, comparative chromosome barcoding was developed using sets of
low-repeat, physically mapped B. distachyon-derived bacterial artificial chromosome (BAC) clones.
• Key Results All species had rather small chromosomes, and essentially all in the Bs genome were
morphometrically indistinguishable. Seven BACs combined with two rDNA-based probes provided unambiguous
and reproducible chromosome discrimination. Comparative chromosome barcoding revealed NCFs that contributed
to the reduction in the x = 12 chromosome number that has been suggested for the intermediate ancestral grass
karyotype. Chromosome Bd3 derives from two NCFs of three ancestral chromosomes (Os2, Os8, Os10).
Chromosome Bs6 shows an ancient Os8/Os10 NCF, whilst Bs4 represents Os2 only. Chromosome Bd4 originated
from a descending dysploidy that involves two NCFs of Os12, Os9 and Os11. The specific distribution of BACs
along Bs9 and Bs5, in both B. stacei and B. hybridum, suggests a Bs genome-specific Robertsonian rearrangement.
• Conclusions mcFISH-based karyotyping identifies all chromosomes in Brachypodium annuals. Comparative
chromosome barcoding reveals rearrangements responsible for the diverse organization of Bd and Bs genomes and
provides new data regarding karyotype evolution since the split of the two diploids. The fact that no chromosome
rearrangements were observed in B. hybridum compared with the karyotypes of its phylogenetic ancestors suggests
prolonged genome stasis after the formation of the allotetraploid. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | Brachypodium | pl_PL |
dc.subject | Brachypodium distachyon | pl_PL |
dc.subject | Brachypodium hybridum | pl_PL |
dc.subject | Brachypodium stacei | pl_PL |
dc.subject | chromosome rearrangements | pl_PL |
dc.subject | comparative chromosome mapping | pl_PL |
dc.subject | dysploidy | pl_PL |
dc.subject | karyotype structure and evolution | pl_PL |
dc.subject | mcFISH | pl_PL |
dc.subject | model grass | pl_PL |
dc.subject | nested chromosome fusions | pl_PL |
dc.subject | Robertsonian rearrangements | pl_PL |
dc.title | Chromosome identification and reconstruction of evolutionary rearrangements in Brachypodium distachyon, B. stacei and B. hybridum | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.1093/aob/mcy086 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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