DC pole | Wartość | Język |
dc.contributor.author | Wójcikowska, Barbara | - |
dc.contributor.author | Botor, Malwina | - |
dc.contributor.author | Morończyk, Joanna | - |
dc.contributor.author | Wójcik, Anna M. | - |
dc.contributor.author | Nodzyński, Tomasz | - |
dc.contributor.author | Karcz, Jagna | - |
dc.contributor.author | Gaj, Małgorzata D. | - |
dc.date.accessioned | 2018-09-27T09:05:59Z | - |
dc.date.available | 2018-09-27T09:05:59Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | Frontiers in Plant Science, Vol. 9 (2018), art. no. 1353 | pl_PL |
dc.identifier.issn | 1664-462X | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/6373 | - |
dc.description.abstract | Auxin is an important regulator of plant ontogenies including embryo development and
the exogenous application of this phytohormone has been found to be necessary for
the induction of the embryogenic response in plant explants that have been cultured
in vitro. However, in the present study, we show that treatment of Arabidopsis explants
with trichostatin A (TSA), which is a chemical inhibitor of histone deacetylases, induces
somatic embryogenesis (SE) without the exogenous application of auxin. We found that
the TSA-treated explants generated somatic embryos that developed efficiently on the
adaxial side of the cotyledons, which are the parts of an explant that are involved in
auxin-induced SE. A substantial reduction in the activity of histone deacetylase (HDAC)
was observed in the TSA-treated explants, thus confirming a histone acetylationrelated
mechanism of the TSA-promoted embryogenic response. Unexpectedly, the
embryogenic effect of TSA was lower on the auxin-supplemented media and this finding
further suggests an auxin-related mechanism of TSA-induced SE. Congruently, we
found a significantly increased content of indolic compounds, which is indicative of IAA
and an enhanced DR5::GUS signal in the TSA-treated explants. In line with these results,
two of the YUCCA genes (YUC1 and YUC10), which are involved in auxin biosynthesis,
were found to be distinctly up-regulated during TSA-induced SE and their expression
was colocalised with the explant sites that are involved in SE. Beside auxin, ROS were
extensively accumulated in response to TSA, thereby indicating that a stress-response
is involved in TSA-triggered SE. Relevantly, we showed that the genes encoding the
transcription factors (TFs) that have a regulatory function in auxin biosynthesis including
LEC1, LEC2, BBM, and stress responses (MYB118) were highly up-regulated in the
TSA-treated explants. Collectively, the results provide several pieces of evidence about
the similarities between the molecular pathways of SE induction that are triggered by
TSA and 2,4-D that involve the activation of the auxin-responsive TF genes that have
a regulatory function in auxin biosynthesis and stress responses. The study suggests
the involvement of histone acetylation in the auxin-mediated release of the embryogenic
program of development in the somatic cells of Arabidopsis. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | Arabidopsis thaliana | pl_PL |
dc.subject | auxin | pl_PL |
dc.subject | epigenetics | pl_PL |
dc.subject | histone acetylation | pl_PL |
dc.subject | in vitro culture | pl_PL |
dc.subject | somatic embryogenesis | pl_PL |
dc.subject | transcription factors | pl_PL |
dc.subject | trichostatin A | pl_PL |
dc.title | Trichostatin A Triggers an Embryogenic Transition in Arabidopsis Explants via an Auxin-Related Pathway | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.3389/fpls.2018.01353 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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