DC pole | Wartość | Język |
dc.contributor.author | Chwiałkowska, Karolina | - |
dc.contributor.author | Korotko, Urszula | - |
dc.contributor.author | Kosińska, Joanna | - |
dc.contributor.author | Szarejko, Iwona | - |
dc.contributor.author | Kwaśniewski, Mirosław | - |
dc.date.accessioned | 2019-03-26T10:33:47Z | - |
dc.date.available | 2019-03-26T10:33:47Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Frontiers in Plant Science, Vol. 8 (2017), Art. No. 2056 | pl_PL |
dc.identifier.issn | 1664-462X | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/8654 | - |
dc.description.abstract | Epigenetic mechanisms, including histone modifications and DNA methylation, mutually
regulate chromatin structure, maintain genome integrity, and affect gene expression
and transposon mobility. Variations in DNA methylation within plant populations, as well
as methylation in response to internal and external factors, are of increasing interest,
especially in the crop research field. Methylation Sensitive Amplification Polymorphism
(MSAP) is one of the most commonly used methods for assessing DNA methylation
changes in plants. This method involves gel-based visualization of PCR fragments
from selectively amplified DNA that are cleaved using methylation-sensitive restriction
enzymes. In this study, we developed and validated a new method based on the
conventional MSAP approach called Methylation Sensitive Amplification Polymorphism
Sequencing (MSAP-Seq). We improved the MSAP-based approach by replacing the
conventional separation of amplicons on polyacrylamide gels with direct, high-throughput
sequencing using Next Generation Sequencing (NGS) and automated data analysis.
MSAP-Seq allows for global sequence-based identification of changes in DNA
methylation. This technique was validated in Hordeum vulgare. However, MSAP-Seq
can be straightforwardly implemented in different plant species, including crops with
large, complex and highly repetitive genomes. The incorporation of high-throughput
sequencing into MSAP-Seq enables parallel and direct analysis of DNA methylation in
hundreds of thousands of sites across the genome. MSAP-Seq provides direct genomic
localization of changes and enables quantitative evaluation. We have shown that the
MSAP-Seqmethod specifically targets gene-containing regions and that a single analysis
can cover three-quarters of all genes in large genomes.Moreover,MSAP-Seq’s simplicity,
cost effectiveness, and high-multiplexing capability make this method highly affordable.
Therefore, MSAP-Seq can be used for DNA methylation analysis in crop plants with large
and complex genomes. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | DNA methylation | pl_PL |
dc.subject | MSAP | pl_PL |
dc.subject | methylome analysis | pl_PL |
dc.subject | new technique | pl_PL |
dc.subject | next generation sequencing | pl_PL |
dc.subject | large genomes | pl_PL |
dc.title | Methylation Sensitive Amplification Polymorphism Sequencing (MSAP-Seq) - A Method for High-Throughput Analysis of Differentially Methylated CCGG Sites in Plants with Large Genomes | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.3389/fpls.2017.02056 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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