Skip navigation

Please use this identifier to cite or link to this item:
Title: Analysis of aluminum toxicity in Hordeum vulgare roots with an emphasis on DNA integrity and cell cycle
Authors: Jaśkowiak, Joanna
Tkaczyk, Oliver
Słota, Michał
Kwaśniewska, Jolanta
Szarejko, Iwona
Keywords: aluminum; cell division; DNA damage; genome; plant; genomic instability; hordeum
Issue Date: 2018
Citation: PLoS ONE, Vol. 13, Issue 2 (2018), art. no. e019315
Abstract: Barley is one of the cereals that are most sensitive to aluminum (Al). Al in acid soils limits barley growth and development and, as a result, its productivity. The inhibition of root growth is a widely accepted indicator of Al stress. Al toxicity is affected by many factors including the culture medium, pH, Al concentration and the duration of the treatment. However, Al can act differently in different species and still Al toxicity in barley deserves study. Since the mechanism of Al toxicity is discussed we cytogenetically describe the effects of different doses of bioavailable Al on the barley nuclear genome—mitotic activity, cell cycle profile and DNA integrity. At the same time, we tested an established deep-water culture (DWC) hydroponics system and analyzed the effects of Al on the root system parameters using WinRHIZO software. We demonstrated the cytotoxic and genotoxic effect of Al in barley root cells. We showed that Al treatment significantly reduced the mitotic activity of the root tip cells and it also induced micronuclei and damaged nuclei. The DNA-damaging effect of Al was observed using the TUNEL test. We define the inhibitory influence of Al on DNA replication in barley. Analysis with the labelling and detection of 5-ethynyl-2‘-deoxyuridin (EdU) showed that the treatment with Al significantly decreased the frequency of S phase cells. We also demonstrated that Al exposure led to changes in the cell cycle profile of barley root tips. The delay of cell divisions observed as increased frequency of cells in G2/M phase after Al treatment was reported using flow cytometry.
DOI: 10.1371/journal.pone.0193156
ISSN: 1932-6203
Appears in Collections:Artykuły (WNP)

Files in This Item:
File Description SizeFormat 
Jaskowiak_Analysis_of_aluminum_toxicity.pdf13,57 MBAdobe PDFView/Open
Show full item record

Uznanie Autorstwa 3.0 Polska Creative Commons License Creative Commons