DC pole | Wartość | Język |
dc.contributor.author | Pilny, Ewelina | - |
dc.contributor.author | Smolarczyk, Ryszard | - |
dc.contributor.author | Jarosz-Biej, Magdalena | - |
dc.contributor.author | Hadyk, Alina | - |
dc.contributor.author | Skorupa, Agnieszka | - |
dc.contributor.author | Ciszek, Mateusz | - |
dc.contributor.author | Kułach, Natalia | - |
dc.contributor.author | Krakowczyk, Łukasz | - |
dc.contributor.author | Gillner, Danuta | - |
dc.contributor.author | Sokół, Maria | - |
dc.contributor.author | Szala, Stanisław | - |
dc.contributor.author | Cichoń, Tomasz | - |
dc.date.accessioned | 2019-03-29T07:53:25Z | - |
dc.date.available | 2019-03-29T07:53:25Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Stem Cell Research & Therapy, Vol. 10 (2019), Art. No. 93 | pl_PL |
dc.identifier.issn | 1757-6512 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/8700 | - |
dc.description.abstract | Background: Adipose-derived mesenchymal stromal cells (ADSCs) are multipotent stromal cells. The cells secrete a
number of cytokines and growth factors and show immunoregulatory and proangiogenic properties. Their properties
may be used to repair damaged tissues. The aim of our work is to explain the muscle damage repair mechanism with the
utilization of the human adipose-derived mesenchymal stromal cells (hADSCs).
Methods: For the hADSCs isolation, we used the subcutaneous adipose tissue collected during the surgery. The murine
hind limb ischemia was used as a model. The unilateral femoral artery ligation was performed on 10–12-week-old male
C57BL/6NCrl and NOD SCID mice. The mice received PBS− (controls) or 1 × 106 hADSCs. One, 3, 7, 14 and 21days after
the surgery, we collected the gastrocnemius muscles for the immunohistochemical analysis. The results were analyzed
with relevant tests using the Statistica software.
Results: The retention time of hADSCs in the limb lasted about 14 days. In the mice receiving hADSCs, the improvement
in the functionality of the damaged limb occurred faster than in the control mice. More new blood vessels were formed
in the limbs of the mice receiving hADSCs than in limbs of the control mice. hADSCs also increased the infiltration of the
macrophages with the M2 phenotype (7-AAD−/CD45+/F4/80+/CD206+) into the ischemic limbs. hADSCs introduced into
the limb of mice secreted interleukin-6. This cytokine stimulates the emergence of the proangiogenic M2 macrophages,
involved, among others, in the repair of a damaged tissue. Both macrophage depletion and IL-6 blockage suppressed the
therapeutic effect of hADSCs. In the mice treated with hADSCs and liposomes with clodronate (macrophages depletion),
the number of capillaries formed was lower than in the mice treated with hADSCs alone. Administration of hADSCs to
the mice that received siltuximab (human IL-6 blocker) did not cause an influx of the M2 macrophages, and the number
of capillaries formed was at the level of the control group, as in contrast to the mice that received only the hADSCs.
Conclusions: The proposed mechanism for the repair of the damaged muscle using hADSCs is based on the activity of
IL-6. In our opinion, the cytokine, secreted by the hADSCs, stimulates the M2 macrophages responsible for repairing
damaged muscle and forming new blood vessels. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/pl/ | * |
dc.subject | hADSCs | pl_PL |
dc.subject | IL-6 | pl_PL |
dc.subject | M2 macrophages | pl_PL |
dc.subject | Blood vessels | pl_PL |
dc.subject | Repairing damaged muscle | pl_PL |
dc.subject | Xenograft | pl_PL |
dc.title | Human ADSC xenograft through IL-6 secretion activates M2 macrophages responsible for the repair of damaged muscle tissue | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.1186/s13287-019-1188-y | - |
Pojawia się w kolekcji: | Artykuły (WNP)
|