DC pole | Wartość | Język |
dc.contributor.author | Borowska, Natalia | - |
dc.contributor.author | Idziak, Dominika | - |
dc.contributor.author | Hasterok, Robert | - |
dc.date.accessioned | 2019-05-06T09:07:57Z | - |
dc.date.available | 2019-05-06T09:07:57Z | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | Chromosome Research, No. 8 (2011), s. 955-967 | pl_PL |
dc.identifier.issn | 0967-3849 | - |
dc.identifier.issn | 1573-6849 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12128/9028 | - |
dc.description.abstract | Sequential immunolocalisation of 5-
methylcytosine (5-MeC) and fluorescence in situ
hybridisation with chromosome-specific BAC clones
were performed on Brachypodium distachyon mitotic
metaphase chromosomes to determine specific DNA
methylation patterns of each chromosome in the
complement. In the majority of cells examined, chromosomes
Bd4 and Bd5, which bear the loci of 5S and
35S ribosomal DNA, respectively, had characteristic 5-
MeC patterns. In contrast, the distribution of 5-MeC
along the metacentric chromosome pairs Bd1, Bd2 and
Bd3 was more variable. There were numerous differences
in distribution of methylated sites between
homologous chromosomes as well as between chromosome
arms. Some chromosome sites, such as pericentromeric
regions, were highly methylated in all
chromosomes. Additionally, the influence of a hypomethylating
agent, 5-azacytidine, on B. distachyon
chromosome methylation patterns was confirmed. It
was found that some chromosome pairs underwent
demethylation more easily than others, but there was
no apparent regularity in demethylation of particular
chromosome segments. | pl_PL |
dc.language.iso | en | pl_PL |
dc.rights | Uznanie autorstwa-Użycie niekomercyjne 3.0 Polska | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/3.0/pl/ | * |
dc.subject | 5-azacytidine | pl_PL |
dc.subject | Brachypodium distachyon | pl_PL |
dc.subject | Chromosome | pl_PL |
dc.subject | DNA methylation | pl_PL |
dc.subject | Epigenetics | pl_PL |
dc.title | DNA methylation patterns of Brachypodium distachyon chromosomes and their alteration by 5-azacytidine treatment | pl_PL |
dc.type | info:eu-repo/semantics/article | pl_PL |
dc.identifier.doi | 10.1007/s10577-011-9243-2 | - |
Pojawia się w kolekcji: | Artykuły (WNP)
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